GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:24678844

From GONUTS
Jump to: navigation, search
Citation

Huang, YJ, Nuckols, JT, Horne, KM, Vanlandingham, D, Lobigs, M and Higgs, S (2014) Mutagenesis analysis of T380R mutation in the envelope protein of yellow fever virus. Virol. J. 11:60

Abstract

The RGD motif in the mosquito-borne flaviviruses envelope protein domain III (EDIII) FG loop was shown to bind negatively charged cellular molecules and mediate virus entry in mammals. However, its importance in virus entry in the mosquito has not yet been defined. The sequences of RGD motifs are conserved in JEV-serocomplex members primarily transmitted by Culex mosquitoes but absent from members of the DENV serocomplex, which utilize Aedes mosquitoes as vectors. Interestingly, the RGD sequence is present in the attenuated 17D strain of yellow fever virus as a result of the T380R mutation in the EDIII of Asibi strain following extensive in vitro passage in mice and chicken embryos and was found to contribute to the more rapid clearance in mice challenged with 17D. However, viral infectivity and dissemination in mosquitoes had not been evaluated for this mutant.

Links

PubMed PMC3974419 Online version:10.1186/1743-422X-11-60

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEFV1:POLG

GO:0019031: viral envelope

ECO:0000315:

C

The infection rate of the 17D/Asibi M-E E T380R mutant (table 1) shows how the T380R mutation in EDIII favored the establishment of the viral infection in Ae. aegypti

complete
CACAO 9908

YEFV1:POLG

part_of

GO:0019031: viral envelope

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

See also

References

See Help:References for how to manage references in GONUTS.