GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:24671056
| Citation |
Hayashi, K, Jutabha, P, Kamai, T, Endou, H and Anzai, N' (2014) LAT1 Is a Central Transporter of Essential Amino Acids in Human Umbilical Vein Endothelial Cells. J. Pharmacol. Sci. ' |
|---|---|
| Abstract |
Endothelial cell proliferation supporting angiogenesis requires sufficient nutrient supply because of facilitated intracellular metabolism. However, little is known about the mechanism for the promotion of nutrient incorporation in proliferating endothelial cells. Here we show that L-type amino acid transporter 1 (LAT1) is a major transporter of essential amino acids in human umbilical vein endothelial cells (HUVECs). Growing HUVECs express a certain level of LAT1. A LAT1-specific inhibitor suppressed leucine uptake, cell proliferation, and tube formation of HUVECs. Therefore, LAT1 acts to support effective uptake of amino acids, which is critical for the optimal function of HUVECs for angiogenesis. |
| Links | |
| Keywords |
|
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0015804: neutral amino acid transport |
ECO:0000315: |
P |
Figure 1B shows how when JPH203, a LAT-1 specific inhibitor was used then the amount of the neutral amino acid leucine was decreased. This shows how when LAT-1 is functional LAT1 acts to support effective uptake of amino acids, which is critical for the optimal function of HUVECs for angiogenesis. |
complete | ||||
|
involved_in |
GO:0015804: neutral amino acid transport |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
