PMID:24634113

Citation	Li, XH, Wu, B, Kong, YB and Zhang, CY (2014) GmPTF1, a novel transcription factor gene, is involved in conferring soybean tolerance to phosphate starvation. Genet. Mol. Res. 13:926-37
Abstract	Phosphorus plays a pivotal role in plant growth and development. In this study, we isolated and characterized GmPTF1, a basic helix-loop-helix (bHLH) transcription factor (TF) gene from soybean (Glycine max) with tolerance to inorganic phosphate (Pi) starvation. Alignment analysis indicated that GmPTF1 and other reported bHLH TFs share significant similarity in the region of the bHLH domain. As with OsPTF1 and other homologous Pi starvation-related bHLH TFs (His-5, Glu-9, Arg-12, and Arg-13), all recognition motifs for the G-box (CACGTG) were present in the GmPTF1 domain. Prokaryotic expression in Escherichia coli strain BL21 (DE3) plysS showed that a novel 40-kDa polypeptide was expressed when cells containing GmPTF1 were induced. The subcellular localization in cells from onion epidermis and Arabidopsis roots demonstrated that the GmPTF1 protein was found in the nucleus. Furthermore, analysis of transcription activity in yeast revealed that full-length GmPTF1 and its N-terminal and C-terminal domains could activate the histidine, adenine, and uracil reporter genes. This suggested that the N-terminal and C-terminal peptides of GmPTF1 act as transcriptional activators. When real-time quantitative polymerase chain reaction was performed, the expression of GmPTF1 under conditions of phosphate starvation was significantly induced in soybean roots of the low-Pi-tolerant variety ZH15. Moreover, the relative level of expression was much higher there than in roots of the sensitive variety NMH from days 7 to 56 of low-Pi stress. These results imply that GmPTF1 is involved in conferring tolerance to phosphate starvation in soybean.
Links	PubMed Online version:10.4238/2014.February.19.3
Keywords
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
SOYBN:C7SQI9		GO:0044798: nuclear transcription factor complex	ECO:0000315:		C		Figure 5: Shows that GmPTF1 is located in the nucleus Figure 6: Used yeast clones and beta-galactosidase assays to show that the N-terminal and C-terminal domains of the full GmPTF1 are used to activate transcription. Only when both are functional can the reporter genes for Ade, Ura and his be activated Figure 7: Shows via qRT-PCR that GmPTF1 expression was induced in soybean varieties more tolerant to phosphate starvation. Time point sampling of plant organs showed that phosphate starvation induced the expression in the tolerant plants. This shows that GmPTF1 is likely a transcription factor involved in phosphate starvation response.	complete CACAO 9674

References

See Help:References for how to manage references in GONUTS.