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PMID:24606679
| Citation |
Huang, Q, Luo, Y, Cheng, F, Best, SM, Bloom, ME and Qiu, J (2014) Molecular characterization of the small nonstructural proteins of parvovirus Aleutian mink disease virus (AMDV) during infection. Virology 452-453:23-31 |
|---|---|
| Abstract |
Aleutian mink disease virus (AMDV) is the only member in genus Amdovirus of the family Parvoviridae. During AMDV infection, six species of viral transcripts are generated from one precursor mRNA through alternative splicing and alternative polyadenylation. In addition to the large non-structural protein NS1, two small non-structural proteins, NS2 and NS3, are putatively encoded (Qiu J, et al., 2006. J. Virol. 80 654-662). However, these two proteins have not been experimentally demonstrated during virus infection, and nothing is known about their function. Here, we studied the nonstructural protein expression profile of AMDV, and for the first time, confirmed expression of NS2 and NS3 during infection, and identified their intracellular localization. More importantly, we provided evidence that both NS2 and NS3 are necessary for AMDV replication. |
| Links |
PubMed PMC3955004 Online version:10.1016/j.virol.2014.01.005 |
| Keywords |
Aleutian Mink Disease/virology; Aleutian Mink Disease Virus/genetics; Aleutian Mink Disease Virus/physiology; Amino Acid Sequence; Animals; Cell Line; Gene Expression Regulation, Viral; Mink; Molecular Sequence Data; Protein Transport; Sequence Alignment; Viral Nonstructural Proteins/genetics; Viral Nonstructural Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0039693: viral DNA genome replication |
ECO:0000315: |
P |
Figure 5 and Figure 6 show that Non-structural protein NS2, or just NS2, is involved in viral genome replication. In the presence of the mutant, pIAMDV(-NS2), the aleutian mink disease parvovirus (strain G) (ADV or AMDV) is unable to replicate its genome within the host cell nucleus. In this experiment, they infected CrFK cells (Crandall feline kidney cell) with the aleutian mink disease parvovirus (AMDV-G) to determine the involvement of NS2 in viral genome replication. |
complete | ||||
| GO:0019034: viral replication complex |
ECO:0000314: |
C |
Figure 4 tells us that Non-Structural Protein NS2, or just NS2, from the aleutian mink disease parvovirus (strain G) (ADV or AMDV) is localized inside the viral DNA replication center of the CrFK host cells (Crandall feline kidney cell). |
complete | ||||
| GO:0019034: viral replication complex |
ECO:0000314: |
C |
Figure 4 tells us that Initiator protein NS1, or just NS1, from the aleutian mink disease parvovirus (strain G) (ADV or AMDV) is localized inside the viral DNA replication center of the CrFK host cells (Crandall feline kidney cell). |
complete | ||||
| GO:0042025: host cell nucleus |
ECO:0000314: |
C |
Figure 4 tells us that Non-Structural Protein NS3, or just NS3, from the aleutian mink disease parvovirus (strain G) (ADV or AMDV) is expressed in the host cell nucleus during AMDV replication but is not localized within the viral DNA replication centers. In this experiment, they infected CrFK cells (Crandall feline kidney cell) with the aleutian mink disease parvovirus (AMDV-G) to determine the involvement of NS3 in viral genome replication. |
complete | ||||
Notes
See also
References
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