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PMID:24592198
| Citation |
Lou, Q, Qi, Y, Ma, Y and Qu, D (2014) Two-component signal transduction system SaeRS positively regulates Staphylococcus epidermidis glucose metabolism. ScientificWorldJournal 2014:908121 |
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| Abstract |
Staphylococcus epidermidis, which is a causative pathogen of nosocomial infection, expresses its virulent traits such as biofilm and autolysis regulated by two-component signal transduction system SaeRS. In this study, we performed a proteomic analysis of differences in expression between the S. epidermidis 1457 wild-type and saeRS mutant to identify candidates regulated by saeRS using two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption/lonization mass spectrometry (MALDI-TOF-MS). Of 55 identified proteins that significantly differed in expression between the two strains, 15 were upregulated and 40 were downregulated. The downregulated proteins included enzymes related to glycolysis and TCA cycle, suggesting that glucose is not properly utilized in S. epidermidis when saeRS was deleted. The study will be helpful for treatment of S. epidermidis infection from the viewpoint of metabolic modulation dependent on two-component signal transduction system SaeRS. |
| Links |
PubMed PMC3921950 Online version:10.1155/2014/908121 |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0010468: regulation of gene expression |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0009386: translational attenuation |
ECO:0000314: |
P |
Figure 4: the biofilm forming ability of WT showed no obvious difference upon the stimulation of glucose. Although it did not reach the level of the wild-type strain, complementation of saeRS resulted in decreased biofilm formation. |
complete | ||||
| GO:0009386: translational attenuation |
ECO:0000315: |
P |
Figure 4: the biofilm forming ability of WT showed no obvious difference upon the stimulation of glucose. Although it did not reach the level of the wild-type strain, complementation of saeRS resulted in decreased biofilm formation. |
complete | ||||
| GO:0010468: regulation of gene expression |
ECO:0000314: |
P |
Figure 3: To verify the differentially expressed proteins regulated by SaeRS at the transcriptional level, glucose metabolism-related genes were chosen for the quantitative real-time RT-PCR analysis. The phosphate acetyltransferase transcription expression levels were significantly upregulated 1.72-fold over than those of the control. |
complete | ||||
See also
References
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