PMID:24586498

Citation	Abiko, Y, Nagano, K, Yoshida, Y and Yoshimura, F (2014) Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola. PLoS ONE 9:e89051
Abstract	The cultivation and genetic manipulation of Treponema denticola, a Gram-negative oral spirochaeta associated with periodontal diseases, is still challenging. In this study, we formulated a simple medium based on a commercially available one, and established a transformation method with high efficiency. We then analyzed proteins in a membrane fraction in T. denticola and identified 16 major membrane-associated proteins, and characterized one of them, TDE2508, whose biological function was not yet known. Although this protein, which exhibited a complex conformation, was presumably localized in the outer membrane, we did not find conclusive evidence that it was exposed on the cell surface. Intriguingly, a TDE2508-deficient mutant exhibited significantly increased biofilm formation and adherent activity on human gingival epithelial cells. However, the protein deficiency did not alter autoaggregation, coaggregation with Porphyromonas gingivalis, hemagglutination, cell surface hydrophobicity, motility, or expression of Msp which was reported to be an adherent molecule in this bacteria. In conclusion, the major membrane protein TDE2508 regulates biofilm formation and the adhesive potency of T. denticola, although the underlying mechanism remains unclear.
Links	PubMed PMC3931704 Online version:10.1371/journal.pone.0089051
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
TREDE:Q73JV3	involved_in	GO:0030155: regulation of cell adhesion	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
TREDE:Q73JV3	involved_in	GO:1900191: negative regulation of single-species biofilm formation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
TREDE:Q73JV3		GO:1900191: negative regulation of single-species biofilm formation	ECO:0000315:		P		In Figure 7 the TDE2508-deficient Mutant and the wild type, T. denticola, were both incubated on a polystyrene plate for 2 days. After two days, the amount of biofilm formation and development of bacterial aggregates were measured. The mutant, which was TDE2508-deficient, had a substantial amount of biofilm formation and bacterial aggregates compared to the wild-type. The mutant's ability to produce more biofilm than the wild-type because of the deletion of TDE2508, supports the claim that TDE2508 is involved in the regulation of biofilm formation.	complete CACAO 10101
TREDE:Q73JV3		GO:0030155: regulation of cell adhesion	ECO:0000315:		P		In Figure 8 the TDE2508-deficient Mutant and the wild type, T. denticola, were both incubated with Ca9-22 epithelial cells for 1 hour. After one hour the Mutant showed significantly more adherence to human gingival epithelial cells at both high and low MOI. Since the figure shows the mutant, which is TDE2508-deficient, as having superior adherence compared to the wild-type, it is clear that the TDE2508 protein plays an important role in regulating cell adhesion.	complete CACAO 10112

References

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