PMID:24534105

Citation	Verma, JK, Gayali, S, Dass, S, Kumar, A, Parveen, S, Chakraborty, S and Chakraborty, N (2014) OsAlba1, a dehydration-responsive nuclear protein of rice (Oryza sativa L. ssp. indica), participates in stress adaptation. Phytochemistry 100:16-25
Abstract	Alba proteins have exhibited great functional plasticity through the course of evolution and constitute a superfamily that spans across three domains of life. Earlier, we had developed the dehydration-responsive nuclear proteome of an indica rice cultivar, screening of which led to the identification of an Alba protein. Here we describe, for the first time, the complete sequence of the candidate gene OsAlba1, its genomic organization, and possible function/s in plant. Phylogenetic analysis showed its close proximity to other monocots as compared to dicot Alba proteins. Protein-DNA interaction prediction indicates a DNA-binding property for OsAlba1. Confocal microscopy showed the localization of OsAlba1-GFP fusion protein to the nucleus, and also sparsely to the cytoplasm. Water-deficit conditions triggered OsAlba1 expression suggesting its function in dehydration stress, possibly through an ABA-dependent pathway. Functional complementation of the yeast mutant ΔPop6 established that OsAlba1 also functions in oxidative stress tolerance. The preferential expression of OsAlba1 in the flag leaves implies its role in grain filling. Our findings suggest that the Alba components such as OsAlba1, especially from a plant where there is no evidence for a major chromosomal role, might play important function in stress adaptation.
Links	PubMed Online version:10.1016/j.phytochem.2014.01.015
Keywords	Active Transport, Cell Nucleus; Adaptation, Physiological; Amino Acid Sequence; Base Sequence; Cell Nucleus/metabolism; Cloning, Molecular; Cytoplasm/metabolism; Evolution, Molecular; Gene Expression Regulation, Plant; Models, Molecular; Molecular Sequence Data; Nuclear Proteins/chemistry; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Oryza/cytology; Oryza/genetics; Oryza/metabolism; Oryza/physiology; Phylogeny; Plant Proteins/chemistry; Plant Proteins/genetics; Plant Proteins/metabolism; Protein Multimerization; Protein Structure, Quaternary; Stress, Physiological; Water/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ORYSI:A0A165TZX1		GO:0005737: cytoplasm	ECO:0000314:		C		As seen by the visualization in figure 6, the OsAlba1 was found in the cytoplasm. Oryza sativa indica.	complete CACAO 12370
ORYSI:A0A165TZX1		GO:0005634: nucleus	ECO:0000314:		C		As visualized in Figure 6, the OsAlba1 protein was fluorescently shown in the nucleus. Oryza sativa indica.	complete CACAO 12371
ORYSI:A0A165TZX1		GO:0006979: response to oxidative stress	ECO:0000316:	UniProtKB:P53218	P		A Pop6 mutant in yeast was complemented by OsAlba1 for oxidative stress tolerance in a strain as seen in Figure 7.	complete CACAO 12372

References

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