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PMID:24288677
| Citation |
Al-Qattan, MM, Shier, MK, Abd-Alwahed, MM, Mawlana, OH, El-Wetidy, MS, Bagayawa, RS, Ali, HH, Al-Nbaheen, MS and Aldahmash, AM (2013) Salamander-derived, human-optimized nAG protein suppresses collagen synthesis and increases collagen degradation in primary human fibroblasts. Biomed Res Int 2013:384091 |
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| Abstract |
Unlike humans, salamanders regrow their amputated limbs. Regeneration depends on the presence of regenerating axons which upregulate the expression of newt anterior gradient (nAG) protein. We had the hypothesis that nAG might have an inhibitory effect on collagen production since excessive collagen production results in scarring, which is a major enemy to regeneration. nAG gene was designed, synthesized, and cloned. The cloned vector was then transfected into primary human fibroblasts. The results showed that the expression of nAG protein in primary human fibroblast cells suppresses the expression of collagen I and III, with or without TGF- β 1 stimulation. This suppression is due to a dual effect of nAG both by decreasing collagen synthesis and by increasing collagen degradation. Furthermore, nAG had an inhibitory effect on proliferation of transfected fibroblasts. It was concluded that nAG suppresses collagen through multiple effects. |
| Links |
PubMed PMC3833026 Online version:10.1155/2013/384091 |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
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Contributes to |
GO:0032966: negative regulation of collagen biosynthetic process |
ECO:0000314: |
P |
Figure 4 displays a western blot assay showing collagen III expression in untreated control fibroblasts and control fibroblasts treated with TGF-β1. With TGF-β1 treatment, there was also decreased collagen III expression in transfected fibroblasts. This shows that the inhibitory effect of nAG is dominant over the TGF-β1 effect. |
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