PMID:24260274

Citation	Yue, L, Guo, S, Zhang, Y, Liu, L, Wang, Q, Wang, X, Shen, D, Wang, L, Sun, L, Wang, J, Liao, Y and Li, Q (2013) The modulation of phosphatase expression impacts the proliferation efficiency of HSV-1 in infected astrocytes. PLoS ONE 8:e79648
Abstract	Herpes Simplex Virus 1 (HSV-1) is a major pathogen that causes human neurological diseases, including herpes simplex encephalitis (HSE). Previous studies have shown that astrocytes are involved in HSV-1 systemic pathogenesis in the central nervous system (CNS), although the mechanism remains unclear. In this study, a high-throughput RNAi library screening method was used to analyze the effect of host phosphatase gene regulation on HSV-1 replication using Macaca mulatta primary astrocytes in an in vitro culture system. The results showed that the downregulation of five phosphatase genes (PNKP, SNAP23, PTPRU, LOC714621 and PPM1M) significantly inhibited HSV-1 infection, suggesting that these phosphatases were needed in HSV-1 replication in rhesus astrocytes. Although statistically significant, the effect of downregulation of these phosphatases on HSV-1 replication in a human astrocytoma cell line appears to be more limited. Our results suggest that the phosphatase genes in astrocytes may regulate the immunological and pathological reactions caused by HSV-1 CNS infection through the regulation of HSV-1 replication or of multiple signal transduction pathways.
Links	PubMed PMC3829861 Online version:10.1371/journal.pone.0079648
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:PTPRU	Contributes to	GO:0016791: phosphatase activity	ECO:0000314:		F		Figure 4: PTPRU genes in astrocytes were identified as key genes affecting HSV-1 replication.	complete CACAO 9369

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