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PMID:24162136
| Citation |
Huang, YJ, Wang, L, Zheng, MG, Zheng, L, Tong, YL and Li, Y (2014) Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation. Biotechnol. Lett. 36:575-9 |
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| Abstract |
Aureochrome-1 (AUREO1) is a transcription factor that is induced by blue light and controls branching of Vaucheria frigida. We have cloned the gene, NgAUREO1, coding for AUREO1 from Nannochloropsis gaditana, and report that the lipid content in recombinant Saccharomyces cerevisiae was 1.6-fold more than in wild-type S. cerevisiae (6.3 % lipid increased to 10 %). Over-expression of AUREO1 in S. cerevisiae up-regulated the expression of acetyl-CoA carboxylase and acyl-CoA:diacylglycerol acyl-transferase but down-regulated the expression of long-chain-acyl CoA synthetase. This enhanced the accumulation of lipid. This study highlights a novel function of AUREO1 and allows a better understanding of the regulation mechanism of fatty acid metabolism. |
| Links |
PubMed Online version:10.1007/s10529-013-1386-0 |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0045723: positive regulation of fatty acid biosynthetic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0045723: positive regulation of fatty acid biosynthetic process |
ECO:0000315: |
P |
Figure 2C shows that when cloned into S. cerevisiae, the protein AUREO1 from N. gaditana significantly increased the amount of lipids in the cell compared to the empty vector. Figure 2D shows that the S. cerevisiae mutant expressing the gene has increased expression of ACC1 and DGAT and decreased expression of LACS, genes that increase and decrease fatty acid production respectively, indicating that the increased lipid contents were due to this regulation. The empty vector did not affect expression of the fatty acid synthesis genes. |
complete | ||||
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