GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:24086592
| Citation |
Lai, D, Tan, CL, Gunaratne, J, Quek, LS, Nei, W, Thierry, F and Bellanger, S (2013) Localization of HPV-18 E2 at mitochondrial membranes induces ROS release and modulates host cell metabolism. PLoS ONE 8:e75625 |
|---|---|
| Abstract |
Papillomavirus E2 proteins are predominantly retained in the nuclei of infected cells, but oncogenic (high-risk) HPV-18 and 16 E2 can shuttle between the host nucleus and cytoplasm. We show here that cytoplasmic HPV-18 E2 localizes to mitochondrial membranes, and independent mass spectrometry analyses of the E2 interactome revealed association to the inner mitochondrial membrane including components of the respiratory chain. Mitochondrial E2 association modifies the cristae morphology when analyzed by electron microscopy and increases production of mitochondrial ROS. This ROS release does not induce apoptosis, but instead correlates with stabilization of HIF-1α and increased glycolysis. These mitochondrial functions are not shared by the non-oncogenic (low-risk) HPV-6 E2 protein, suggesting that modification of cellular metabolism by high-risk HPV E2 proteins could play a role in carcinogenesis by inducing the Warburg effect. |
| Links |
PubMed PMC3782431 Online version:10.1371/journal.pone.0075625 |
| Keywords |
Apoptosis/physiology; Cell Line, Tumor; Cell Nucleus/metabolism; Cell Nucleus/virology; Cytoplasm/metabolism; Cytoplasm/virology; Glycolysis/physiology; Human papillomavirus 18/metabolism; Humans; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism; Mitochondria/metabolism; Mitochondria/virology; Mitochondrial Membranes/metabolism; Mitochondrial Membranes/virology; Oncogene Proteins, Viral/metabolism; Reactive Oxygen Species/metabolism; Viral Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0044191: host cell mitochondrial membrane |
ECO:0000314: |
C |
Figure 3: "HPV-18 E2 localizes to mitochondrial membranes" Regulatory protein E2 Human papillomavirus type 18 |
complete | ||||
| GO:0044193: host cell mitochondrial outer membrane |
ECO:0000314: |
C |
Mass spec analyses revealed association of E2 with outer mitochondrial membrane, as seen on FIG 2 and FIG 3. Regulatory protein E2 Human papillomavirus type 18 |
complete | ||||
Notes
See also
References
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