PMID:23870126

Citation	Subramanian, R, Ti, SC, Tan, L, Darst, SA and Kapoor, TM (2013) Marking and measuring single microtubules by PRC1 and kinesin-4. Cell 154:377-90
Abstract	Error-free cell division depends on the assembly of the spindle midzone, a specialized array of overlapping microtubules that emerges between segregating chromosomes during anaphase. The molecular mechanisms by which a subset of dynamic microtubules from the metaphase spindle are selected and organized into a stable midzone array are poorly understood. Here, we show using in vitro reconstitution assays that PRC1 and kinesin-4, two microtubule-associated proteins required for midzone assembly, can tag microtubule plus ends. Remarkably, the size of these tags is proportional to filament length. We determine the crystal structure of the PRC1 homodimer and map the protein-protein interactions needed for tagging microtubule ends. Importantly, length-dependent microtubule plus-end-tagging by PRC1 is also observed in dividing cells. Our findings suggest how biochemically similar microtubules can be differentially marked, based on length, for selective regulation during the formation of specialized arrays, such as those required for cytokinesis.
Links	PubMed PMC3761943 Online version:10.1016/j.cell.2013.06.021
Keywords	Amino Acid Sequence; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/metabolism; Crystallography, X-Ray; Cytokinesis; Dimerization; Humans; Kinesin/metabolism; Microtubules/metabolism; Models, Molecular; Molecular Sequence Data; Sequence Alignment
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:PRC1	enables	GO:0042802: identical protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O43663-1	F		Seeded From UniProt	complete

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