PMID:23867456

Citation	Boggaram, V, Gottipati, KR, Wang, X and Samten, B (2013) Early secreted antigenic target of 6 kDa (ESAT-6) protein of Mycobacterium tuberculosis induces interleukin-8 (IL-8) expression in lung epithelial cells via protein kinase signaling and reactive oxygen species. J. Biol. Chem. 288:25500-11
Abstract	Early secreted antigenic target of 6 kDa (ESAT-6) of Mycobacterium tuberculosis is critical for the virulence and pathogenicity of M. tuberculosis. IL-8, a major chemotactic cytokine for neutrophils and T lymphocytes, plays important roles in the development of lung injury. To further understand the role of ESAT-6 in lung pathology associated with tuberculosis development, we studied the effects of ESAT-6 on the regulation of IL-8 expression in lung epithelial cells. ESAT-6 induced IL-8 expression by increasing IL-8 gene transcription and mRNA stability. ESAT-6 induction of IL-8 promoter activity was dependent on nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) binding and sensitive to pharmacological inhibition of PKC and ERK and p38 MAPK pathways. ESAT-6 activated ERK and p38 MAPK phosphorylation and rapidly induced reactive oxygen species (ROS) production. Dimethylthiourea but not mannitol inhibited IL-8 induction by ESAT-6, further supporting the involvement of ROS in the induction of IL-8 expression. Exposure of mice to ESAT-6 induced localized inflammatory cell aggregate formation with characteristics of early granuloma concomitant with increased keratinocyte chemoattractant CXCL1 staining in bronchiolar and alveolar type II epithelial cells and alveolar macrophages. Our studies have identified a signal transduction pathway involving ROS, PKC, ERK, and p38 MAPKs and NF-κB and AP-1 in the ESAT-6 induction of IL-8 expression in lung epithelial cells. This has important implications for the understanding of lung innate immune responses to tuberculosis and the pathogenesis of lung injury in tuberculosis.
Links	PubMed PMC3757211 Online version:10.1074/jbc.M112.448217
Keywords	Animals; Antigens, Bacterial/metabolism; Antigens, Bacterial/pharmacology; Bacterial Proteins/metabolism; Bacterial Proteins/pharmacology; Cell Line, Tumor; Epithelial Cells/metabolism; Epithelial Cells/pathology; Gene Expression Regulation; Humans; Interleukin-8/biosynthesis; Interleukin-8/genetics; Lung/metabolism; Lung/pathology; MAP Kinase Signaling System; Macrophages, Alveolar/metabolism; Macrophages, Alveolar/pathology; Mice; Mycobacterium tuberculosis/metabolism; NF-kappa B/genetics; NF-kappa B/metabolism; Promoter Regions, Genetic/genetics; Protein Kinases/genetics; Protein Kinases/metabolism; Reactive Oxygen Species/metabolism; Respiratory Mucosa/metabolism; Respiratory Mucosa/pathology; Transcription Factor AP-1/genetics; Transcription Factor AP-1/metabolism; Transcription, Genetic/genetics; Tuberculosis, Pulmonary/genetics; Tuberculosis, Pulmonary/metabolism; Tuberculosis, Pulmonary/pathology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MYCTU:ESXA		GO:0032757: positive regulation of interleukin-8 production	ECO:0000314:		P		in Figure 1E,H, Introduction of Esat-6 increased Interleukin-8 protein levels.	complete CACAO 11398

References

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