PMID:23671330

Citation	Huang, W, Chen, Q, Zhu, Y, Hu, F, Zhang, L, Ma, Z, He, Z and Huang, J' (2013) Arabidopsis Thylakoid Formation 1 Is a Critical Regulator for Dynamics of PSII-LHCII Complexes in Leaf Senescence and Excess Light. Mol Plant '
Abstract	In higher plants, photosystem II (PSII) is a large pigment-protein supramolecular complex composed of the PSII core complex and the plant-specific peripheral light-harvesting complexes (LHCII). PSII-LHCII complexes are highly dynamic in their quantity and macro-organization to various environmental conditions. In this study, we reported a novel factor, the Arabidopsis Thylakoid Formation 1 (THF1) protein, which controls PSII-LHCII dynamics during dark-induced senescence and light acclimation. Loss-of-function mutations in THF1 lead to a stay-green phenotype in pathogen-infected and senescent leaves. Both LHCII and PSII core subunits are retained in dark-induced senescent leaves of thf1, indicative of the presence of PSII-LHCII complexes. Blue-Native (BN)-polyacrylamide gel electrophoresis (PAGE) and immunoblot analysis showed that in dark- and high-light-treated thf1 leaves, a type of PSII-LHCII megacomplex is selectively retained while the stability of PSII-LHCII supercomplexes significantly decreased, suggesting a dual role of THF1 in dynamics of PSII-LHCII complexes. We showed further that THF1 interacts with Lhcb proteins in a pH-dependent manner and that the stay-green phenotype of thf1 relies on the presence of LHCII complexes. Taken together, the data suggest that THF1 is required for dynamics of PSII-LHCII supramolecular organization in higher plants.
Links	PubMed Online version:10.1093/mp/sst069
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:THF1		GO:0015996: chlorophyll catabolic process	ECO:0000315:		P		Figure 1	complete CACAO 8442
ARATH:THF1	involved_in	GO:0015996: chlorophyll catabolic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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