PMID:23499247

Citation	Eikenes, ÅH, Brech, A, Stenmark, H and Haglund, K (2013) Spatiotemporal control of Cindr at ring canals during incomplete cytokinesis in the Drosophila male germline. Dev. Biol. 377:9-20
Abstract	During male and female gametogenesis in species ranging from insects to mammals, germ cell cyst formation by incomplete cytokinesis involves the stabilization of cleavage furrows and the formation of stable intercellular bridges called ring canals. Accurate regulation of incomplete cytokinesis is required for both female and male fertility in Drosophila melanogaster. Nevertheless, the molecular mechanisms controlling complete versus incomplete cytokinesis are largely unknown. Here, we show that the scaffold protein Cindr is a novel component of both mitotic and meiotic ring canals during Drosophila spermatogenesis. Strikingly, unlike other male germline ring canal components, including Anillin and Pavarotti, Cindr and contractile ring F-actin dissociate from mitotic ring canals and translocate to the fusome upon completion of the mitotic germ cell divisions. We provide evidence that the loss of Cindr from mitotic ring canals is coordinated by signals that mediate the transition from germ cell mitosis to differentiation. Interestingly, Cindr loss from ring canals coincides with completion of the mitotic germ cell divisions in both Drosophila females and males, thus marking a common step of gametogenesis. We also show that Cindr co-localizes with Anillin at mitotic and meiotic ring canals and is recruited to the contractile ring by Anillin during male germ cell meiotic cytokinesis. Taken together, our analyses reveal a key step of incomplete cytokinesis at the endpoint of the mitotic germ cell divisions in D. melanogaster.
Links	PubMed Online version:10.1016/j.ydbio.2013.02.021
Keywords	Animals; Cell Differentiation; Cell Fusion; Cell Membrane/metabolism; Contractile Proteins/metabolism; Cytokinesis; Drosophila Proteins/chemistry; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/metabolism; Female; Germ Cells/cytology; Germ Cells/metabolism; Male; Meiosis; Microfilament Proteins/chemistry; Microfilament Proteins/metabolism; Microtubule-Associated Proteins/metabolism; Mitosis; Models, Biological; Protein Transport; Spermatocytes/cytology; Spermatocytes/metabolism; Spermatogenesis; Testis/cytology; Time Factors
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DROME:Q7YU85		GO:0045169: fusome	ECO:0000314:		C		Organism: Drosophila male Protein: Q7YU85 cindr Figure 3. B, C. Cindr translocates to the fusome of maturing primary spermatocytes. n. (B) Magnification of the area indicated in (A) shows a weak band of Cindr. (C)–(C00) Immunostaining wild-type males for Cindr (green) and Phalloidin to visualize F-actin (red) reveals a band of Cindr co-localizing with the fusome (arrows) during the primary spermatocyte growth phase between the ring canals, highlighted by arrows, and indicates translocation to the fusome	complete CACAO 12341

References

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