PMID:23482306

Citation	Yan, J, Chao, DL, Toba, S, Koyasako, K, Yasunaga, T, Hirotsune, S and Shen, K (2013) Kinesin-1 regulates dendrite microtubule polarity in Caenorhabditis elegans. Elife 2:e00133
Abstract	In neurons, microtubules (MTs) span the length of both axons and dendrites, and the molecular motors use these intracellular 'highways' to transport diverse cargo to the appropriate subcellular locations. Whereas axonal MTs are organized such that the plus-end is oriented out from the cell body, dendrites exhibit a mixed MTs polarity containing both minus-end-out and plus-end-out MTs. The molecular mechanisms underlying this differential organization, as well as its functional significance, are unknown. Here, we show that kinesin-1 is critical in establishing the characteristic minus-end-out MT organization of the dendrite in vivo. In unc-116 (kinesin-1/kinesin heavy chain) mutants, the dendritic MTs adopt an axonal-like plus-end-out organization. Kinesin-1 protein is able to cross-link anti-paralleled MTs in vitro. We propose that kinesin-1 regulates the dendrite MT polarity through directly gliding the plus-end-out MTs out of the dendrite using both the motor domain and the C-terminal MT-binding domain. DOI:http://dx.doi.org/10.7554/eLife.00133.001.
Links	PubMed PMC3591006 Online version:10.7554/eLife.00133
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CAEEL:KINH		GO:0030951: establishment or maintenance of microtubule cytoskeleton polarity	ECO:0000315:		P		Figure 1.	complete CACAO 8311
CAEEL:KINH	involved_in	GO:0030951: establishment or maintenance of microtubule cytoskeleton polarity	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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