PMID:23460907

Citation	Ling, J, Pan, H, Gao, Q, Xiong, L, Zhou, Y, Zhang, D, Gao, S and Liu, X (2013) Aerobactin synthesis genes iucA and iucC contribute to the pathogenicity of avian pathogenic Escherichia coli O2 strain E058. PLoS ONE 8:e57794
Abstract	Aerobactin genes are known to be present in virulent strains and absent from avirulent strains, but contributions of iucC and iucA, which are involved in aerobactin synthesis, to the pathogenicity of avian pathogenic Escherichia coli (APEC) have not been clarified. In this study, effects of double mutants (iucA/iutA or iucC/iutA) compared to those of single mutants (iucA, iucC or iutA) of aerobactin genes on the virulence of APEC strain E058 were examined both in vitro (aerobactin production, ingestion into HD-11 cells, survival in chicken serum) and in vivo (competitive growth against parental strain, colonization and persistence). In competitive co-infection assays, compared to the E058 parental strain, the E058ΔiucA mutant was significantly reduced in the liver, kidney, spleen (all P<0.01), heart and lung (both P<0.001). The E058ΔiutA mutant also was significantly reduced in the liver, lung, kidney (all P<0.01), heart and spleen (both P<0.001). The E058ΔiucC mutant was significantly attenuated in the heart and kidney (both P<0.05) and showed a remarkable reduction in the liver, spleen and lung (P<0.01); meanwhile, both E058ΔiucAΔiutA and E058ΔiucCΔiutA double mutants were sharply reduced as well (P<0.001). In colonization and persistence assays, compared with E058, recovered colonies of E058ΔiucA were significantly reduced from the lung, liver, spleen and kidney (P<0.01) and significantly reduced in the heart (P<0.001). E058ΔiutA was significantly reduced from the heart, lung, liver, spleen and kidney (P<0.01). E058ΔiucC, E058ΔiucAΔiutA and E058ΔiucCΔiutA were significantly decreased in all organs tested (P<0.001). These results suggest that iutA, iucA and iucC play important roles in the pathogenicity of APEC E058.
Links	PubMed PMC3584046 Online version:10.1371/journal.pone.0057794
Keywords	Animals; Chickens/blood; Chickens/microbiology; Colony Count, Microbial; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/pathogenicity; Escherichia coli Infections/genetics; Escherichia coli Infections/microbiology; Escherichia coli Infections/veterinary; Gene Deletion; Gene Expression Regulation, Bacterial; Genes, Bacterial/genetics; Genetic Complementation Test; Hydroxamic Acids/metabolism; Poultry Diseases/microbiology; Specific Pathogen-Free Organisms; Virulence/genetics
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLX:IUCA		GO:0009405: pathogenesis	ECO:0000315:		P		Figure 5. Colonization and persistence of wild-type strain Escherichia coli E058 and its mutant E058∆ iucA. The ability of E058 ∆iucA to colonize was significantly less than the wild-type in tissue samples from an individual chicken	complete CACAO 12393

References

See Help:References for how to manage references in GONUTS.