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PMID:23402750

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Citation

Huang, F, Xiao, H, Sun, BL and Yang, RG (2013) Characterization of TRIM62 as a RING finger E3 ubiquitin ligase and its subcellular localization. Biochem. Biophys. Res. Commun. 432:208-13

Abstract

TRIM62, also named DEAR1, is a member of the TRIM/RBCC family, which includes proteins with conserved RING finger, B-box and coiled-coil domains. Several reports have identified a role for this family in cancer, retroviral infection and innate immunity. In this study, the E3 ubiquitin ligase activity and subcellular localization of TRIM62 were characterized. TRIM62, in association with the E2 enzyme UbcH5b, was found to catalyze self-ubiquitination in vitro, a process that required an intact RING finger domain. A ubiquitination assay performed in HEK293T cells further confirmed the E3 ubiquitin ligase activity and self-ubiquitination activity of TRIM62 and the requirement of the RING finger domain. Importantly, the treatment of HEK293T cells with a proteasome inhibitor stabilized poly-ubiquitinated TRIM62, indicating that self-ubiquitination promoted the proteasomal degradation of TRIM62. Additionally, TRIM62 and its two mutants were distinctly localized in the cytoplasm in both HEK293T and HeLa cells. Collectively, our data indicate that TRIM62, a cytoplasmic protein, is a RING finger domain-dependent E3 ubiquitin ligase that catalyzes self-ubiquitination both in vitro and in vivo.

Links

PubMed Online version:10.1016/j.bbrc.2013.02.012

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:TRI62

GO:0005737: cytoplasm

ECO:0000314:

C

Figure 4.

complete
CACAO 8254

HUMAN:TRI62

GO:0004842: ubiquitin-protein ligase activity

ECO:0000314:

F

Figure 1A.

complete
CACAO 8255

HUMAN:TRI62

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:TRI62

enables

GO:0004842: ubiquitin-protein transferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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