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PMID:23353999
| Citation |
Das, S, Cong, R, Shandilya, J, Senapati, P, Moindrot, B, Monier, K, Delage, H, Mongelard, F, Kumar, S, Kundu, TK and Bouvet, P (2013) Characterization of nucleolin K88 acetylation defines a new pool of nucleolin colocalizing with pre-mRNA splicing factors. FEBS Lett. 587:417-24 |
|---|---|
| Abstract |
Nucleolin is a multifunctional protein that carries several post-translational modifications. We characterized nucleolin acetylation and developed antibodies specific to nucleolin K88 acetylation. Using this antibody we show that nucleolin is acetylated in vivo and is not localized in the nucleoli, but instead is distributed throughout the nucleoplasm. Immunofluorescence studies indicate that acetylated nucleolin is co-localized with the splicing factor SC35 and partially with Y12. Acetylated nucleolin is expressed in all tested proliferating cell types. Our findings show that acetylation defines a new pool of nucleolin which support a role for nucleolin in the regulation of mRNA maturation and transcription by RNA polymerase II. |
| Links |
PubMed Online version:10.1016/j.febslet.2013.01.035 |
| Keywords |
Acetylation; Amino Acid Sequence; Animals; Antibody Specificity; Blotting, Western; Cell Nucleolus/metabolism; Cell Nucleus/metabolism; HeLa Cells; Humans; Immune Sera/chemistry; Leukocytes, Mononuclear/metabolism; Lysine/chemistry; Lysine/immunology; Lysine/metabolism; Molecular Sequence Data; Peptide Fragments/chemistry; Peptide Fragments/immunology; Phosphoproteins/chemistry; Phosphoproteins/immunology; Phosphoproteins/metabolism; Protein Processing, Post-Translational; Protein Transport; RNA-Binding Proteins/chemistry; RNA-Binding Proteins/immunology; RNA-Binding Proteins/metabolism; Rabbits; Ribonucleoproteins, Small Nuclear/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0005654: nucleoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0005654: nucleoplasm |
ECO:0000314: |
C |
Figure 3. |
complete | ||||
See also
References
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