PMID:23341464

Citation	Agarwal, V, Kuchipudi, A, Fulde, M, Riesbeck, K, Bergmann, S and Blom, AM (2013) Streptococcus pneumoniae endopeptidase O (PepO) is a multifunctional plasminogen- and fibronectin-binding protein, facilitating evasion of innate immunity and invasion of host cells. J. Biol. Chem. 288:6849-63
Abstract	Streptococcus pneumoniae infections remain a major cause of morbidity and mortality worldwide. Therefore a detailed understanding and characterization of the mechanism of host cell colonization and dissemination is critical to gain control over this versatile pathogen. Here we identified a novel 72-kDa pneumococcal protein endopeptidase O (PepO), as a plasminogen- and fibronectin-binding protein. Using a collection of clinical isolates, representing different serotypes, we found PepO to be ubiquitously present both at the gene and protein level. In addition, PepO protein was secreted in a growth phase-dependent manner to the culture supernatants of the pneumococcal isolates. Recombinant PepO bound human plasminogen and fibronectin in a dose-dependent manner and plasminogen did not compete with fibronectin for binding PepO. PepO bound plasminogen via lysine residues and the interaction was influenced by ionic strength. Moreover, upon activation of PepO-bound plasminogen by urokinase-type plasminogen activator, generated plasmin cleaved complement protein C3b thus assisting in complement control. Furthermore, direct binding assays demonstrated the interaction of PepO with epithelial and endothelial cells that in turn blocked pneumococcal adherence. Moreover, a pepO-mutant strain showed impaired adherence to and invasion of host cells compared with their isogenic wild-type strains. Taken together, the results demonstrated that PepO is a ubiquitously expressed plasminogen- and fibronectin-binding protein, which plays role in pneumococcal invasion of host cells and aids in immune evasion.
Links	PubMed PMC3591595 Online version:10.1074/jbc.M112.405530
Keywords	Bacterial Adhesion/genetics; Bacterial Adhesion/immunology; Bacterial Proteins/genetics; Bacterial Proteins/immunology; Bacterial Proteins/metabolism; Blotting, Western; Cell Line, Tumor; Cells, Cultured; Complement C3b/immunology; Complement C3b/metabolism; Endopeptidases/genetics; Endopeptidases/immunology; Endopeptidases/metabolism; Epithelial Cells/immunology; Epithelial Cells/metabolism; Epithelial Cells/microbiology; Fibrinolysin/immunology; Fibrinolysin/metabolism; Fibronectins/immunology; Fibronectins/metabolism; Human Umbilical Vein Endothelial Cells/immunology; Human Umbilical Vein Endothelial Cells/metabolism; Human Umbilical Vein Endothelial Cells/microbiology; Humans; Immune Evasion/immunology; Immunity, Innate/immunology; Microscopy, Confocal; Mutation; Plasminogen/immunology; Plasminogen/metabolism; Protein Binding; Streptococcus pneumoniae/genetics; Streptococcus pneumoniae/immunology; Streptococcus pneumoniae/metabolism; Urokinase-Type Plasminogen Activator/immunology; Urokinase-Type Plasminogen Activator/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
STRR6:Q8DNW9	Contributes to	GO:0098635: protein complex involved in cell-cell adhesion	ECO:0000314:		C		Figure 9. Charts B, C, and D indicate bacteria lacking PepO gene had significantly less adherence, thus reducing rates of cell invasion and bacteria survival.	complete CACAO 11348

References

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