PMID:23291412

Citation	Wang, R, Gao, H, Xu, W, Li, H, Mao, Y, Wang, Y, Guo, T, Wang, X, Song, R, Li, Z, Irwin, DM, Niu, G and Tan, H (2013) Differential expression of genes and changes in glucose metabolism in the liver of liver-specific glucokinase gene knockout mice. Gene 516:248-54
Abstract	To investigate the role of liver-specific expression of glucokinase (GCK) in the pathogenesis of hyperglycemia and to identify candidate genes involved in mechanisms of the onset and progression of maturity onset diabetes of the young, type 2 (MODY-2), we examined changes in biochemical parameters and gene expression in GCK knockout (gck(w/-)) and wild-type (gck(w/w)) mice as they aged. Fasting blood glucose levels were found to be significantly higher in the gck(w/-) mice, compared to age-matched gck(w/w) mice, at all ages (P<0.05), except at 2 weeks. GCK activity of gck(w/-) mice was about 50% of that of wild type (gck(w/w)) mice (P<0.05). Glycogen content at 4 and 40 weeks of age was lower in gck(w/-) mice compared to gck(w/w) mice. Differentially expressed genes in the livers of 2 and 26 week-old liver-specific GCK knockout (gck(w/-)) mice were identified by suppression subtractive hybridization (SSH), which resulted in the identification of phosphoenolpyruvatecarboxykinase (PEPCK, also called PCK1) and Sterol O-acyltransferase 2 (SOAT2) as candidate genes involved in pathogenesis. The expressions of PEPCK and SOAT2 along with glycogen phosphorylase (GP) and glycogen synthase (GS) were then examined in GCK knockout (gck(w/-)) and wild-type (gck(w/w)) mice at different ages. Changes in PEPCK mRNA levels were confirmed by real-time RT-PCR, while no differences in the levels of expression of SOAT2 or GS were observed in age-matched GCK knockout (gck(w/-)) and wild-type (gck(w/w)) mice. GP mRNA levels were decreased in 40-week old gck(w/-) mice compared to age-matched gck(w/w) mice. Changes in gluconeogenesis, delayed development of GCK and impaired hepatic glycogen synthesis in the liver potentially lead to the onset and progression of MODY2.
Links	PubMed PMC3642073 Online version:10.1016/j.gene.2012.12.036
Keywords	Age Factors; Animals; Carbohydrate Metabolism/genetics; Diabetes Mellitus, Type 2/genetics; Diabetes Mellitus, Type 2/metabolism; Diabetes Mellitus, Type 2/pathology; Disease Models, Animal; Disease Progression; Gene Expression Regulation; Glucokinase/genetics; Glucokinase/metabolism; Gluconeogenesis/genetics; Gluconeogenesis/physiology; Glucose/metabolism; Liver/metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Organ Specificity/genetics; Transcriptome
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:Q5SVI6		GO:0004340: glucokinase activity	ECO:0000315:		F		Figure 1 a. shows higher blood glucose levels in mice with the Gck knockdown then in those mice with wild type Gck.	complete CACAO 10091

References

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