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PMID:23164714
| Citation |
Qiu, Y, Lee, KS, Choo, YM, Kong, D, Yoon, HJ and Jin, BR (2013) Molecular cloning and antifibrinolytic activity of a serine protease inhibitor from bumblebee (Bombus terrestris) venom. Toxicon 63:1-6 |
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| Abstract |
Bumblebee (Bombus spp.) venom contains a variety of components, including bombolitin, phospholipase A(2) (PLA(2)), serine proteases, and serine protease inhibitors. In this study, we identified a bumblebee (Bombus terrestris) venom serine protease inhibitor (Bt-KTI) that acts as a plasmin inhibitor. Bt-KTI consists of a 58-amino acid mature peptide that displays features consistent with snake venom Kunitz-type inhibitors, including six conserved cysteine residues and a P1 site. Recombinant Bt-KTI was expressed as a 6.5-kDa peptide in baculovirus-infected insect cells. The recombinant peptide demonstrated properties similar to Kunitz-type trypsin inhibitors. Bt-KTI showed no detectable inhibitory effects on factor Xa, thrombin, or tissue plasminogen activator; however, Bt-KTI strongly inhibited plasmin, indicating that it acts as an antifibrinolytic agent. These findings demonstrate the antifibrinolytic role of Bt-KTI as a plasmin inhibitor. |
| Links |
PubMed Online version:10.1016/j.toxicon.2012.11.004 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0004867: serine-type endopeptidase inhibitor activity |
ECO:0000314: |
F |
Figure 2B. Bt-KTI inhibits the enzymatic activity of trypsin |
complete | ||||
| GO:0051918: negative regulation of fibrinolysis |
ECO:0000314: |
P |
Fig 3: Bt-KTI inhibits plasmin formation |
complete | ||||
|
enables |
GO:0004867: serine-type endopeptidase inhibitor activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051918: negative regulation of fibrinolysis |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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