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PMID:23010168

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Citation

Wang, T, Yu, B, Lin, L, Zhai, X, Han, Y, Qin, Y, Guo, Z, Wu, S, Zhong, X, Wang, Y, Tong, L, Zhang, F, Si, X, Zhao, W and Zhong, Z (2012) A functional nuclear localization sequence in the VP1 capsid protein of coxsackievirus B3. Virology 433:513-21

Abstract

The capsid proteins of some RNA viruses can translocate to the nucleus and interfere with cellular phenotypes. In this study we found that the VP1 capsid protein of coxsackievirus B3 (CVB3) was dominantly localized in the nucleus of the cells transfected with VP1-expressing plasmid. The VP1 nuclear localization also occurred in the cells infected with CVB3. Truncation analysis indicated that the VP1 nuclear localization sequence located near the C-terminal. The substitution of His220 with threonine completely abolished its translocation. The VP1 proteins of other CVB types might have the nuclear localization potential because this region was highly conserved. Moreover, the VP1 nuclear localization induced cell cycle deregulation, including a prolonged S phase and shortened G2-M phase. Besides these findings, we also found a domain between Ala72 and Phe106 that caused the VP1 truncates dotted distributed in the cytoplasm. Our results suggest a new pathogenic mechanism of CVB.

Links

PubMed Online version:10.1016/j.virol.2012.08.040

Keywords

Amino Acid Sequence; Amino Acid Substitution; Cell Cycle; Cell Nucleus/metabolism; Cell Nucleus/virology; Enterovirus B, Human/genetics; Enterovirus B, Human/metabolism; Enterovirus B, Human/pathogenicity; HeLa Cells; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Nuclear Localization Signals/genetics; Nuclear Localization Signals/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Sequence Homology, Amino Acid; Transfection; Viral Structural Proteins/genetics; Viral Structural Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CXB3N:POLG

GO:0042025 : host cell nucleus

ECO:0000314:

C

Fig. 1 shows the nuclear localization of this protein

complete
CACAO 6334

CXB3N:POLG

GO:0042025 : host cell nucleus

ECO:0000315:

C

Fig. 4 shows the effects of mutation of the nuclear localization signal

complete
CACAO 6335

CXB3N:POLG

part_of

GO:0042025: host cell nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CXB3N:POLG

part_of

GO:0042025: host cell nucleus

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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