PMID:23010168

Citation	Wang, T, Yu, B, Lin, L, Zhai, X, Han, Y, Qin, Y, Guo, Z, Wu, S, Zhong, X, Wang, Y, Tong, L, Zhang, F, Si, X, Zhao, W and Zhong, Z (2012) A functional nuclear localization sequence in the VP1 capsid protein of coxsackievirus B3. Virology 433:513-21
Abstract	The capsid proteins of some RNA viruses can translocate to the nucleus and interfere with cellular phenotypes. In this study we found that the VP1 capsid protein of coxsackievirus B3 (CVB3) was dominantly localized in the nucleus of the cells transfected with VP1-expressing plasmid. The VP1 nuclear localization also occurred in the cells infected with CVB3. Truncation analysis indicated that the VP1 nuclear localization sequence located near the C-terminal. The substitution of His220 with threonine completely abolished its translocation. The VP1 proteins of other CVB types might have the nuclear localization potential because this region was highly conserved. Moreover, the VP1 nuclear localization induced cell cycle deregulation, including a prolonged S phase and shortened G2-M phase. Besides these findings, we also found a domain between Ala72 and Phe106 that caused the VP1 truncates dotted distributed in the cytoplasm. Our results suggest a new pathogenic mechanism of CVB.
Links	PubMed Online version:10.1016/j.virol.2012.08.040
Keywords	Amino Acid Sequence; Amino Acid Substitution; Cell Cycle; Cell Nucleus/metabolism; Cell Nucleus/virology; Enterovirus B, Human/genetics; Enterovirus B, Human/metabolism; Enterovirus B, Human/pathogenicity; HeLa Cells; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Nuclear Localization Signals/genetics; Nuclear Localization Signals/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Sequence Homology, Amino Acid; Transfection; Viral Structural Proteins/genetics; Viral Structural Proteins/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CXB3N:POLG		GO:0042025 : host cell nucleus	ECO:0000314:		C		Fig. 1 shows the nuclear localization of this protein	complete CACAO 6334
CXB3N:POLG		GO:0042025 : host cell nucleus	ECO:0000315:		C		Fig. 4 shows the effects of mutation of the nuclear localization signal	complete CACAO 6335
CXB3N:POLG	part_of	GO:0042025: host cell nucleus	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
CXB3N:POLG	part_of	GO:0042025: host cell nucleus	ECO:0000315: mutant phenotype evidence used in manual assertion		C		Seeded From UniProt	complete

References

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