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PMID:22817265
| Citation |
Bhubhanil, S, Ruangkiattikul, N, Niamyim, P, Chamsing, J, Ngok-Ngam, P, Sukchawalit, R and Mongkolsuk, S (2012) Identification of amino acid residues important for the function of Agrobacterium tumefaciens Irr protein. FEMS Microbiol. Lett. 335:68-77 |
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| Abstract |
The key amino acid residues that influence the function of the Agrobacterium tumefaciens iron response regulator protein (Irr(At) ) were investigated. Several Irr(At) mutant proteins containing substitutions in amino acids corresponding to candidate metal- and haem-binding sites were constructed. The ability of the mutant proteins to repress the promoter of the membrane bound ferritin (mbfA) gene was investigated using a promoter-lacZ fusion assay. A single mutation at residue H94 significantly decreased the repressive activity of Irr(At) . Multiple mutation analysis revealed the importance of H45, H65, the HHH motif (H92, H93 and H94) and H127 for the repressor function of Irr(At) . H94 is essential for the iron responsiveness of Irr(At) . Furthermore, the Irr(At) mutant proteins showed differential abilities to complement the H(2) O(2) -hyper-resistant phenotype of an irr mutant. |
| Links |
PubMed Online version:10.1111/j.1574-6968.2012.02638.x |
| Keywords |
Agrobacterium tumefaciens/chemistry; Agrobacterium tumefaciens/genetics; Agrobacterium tumefaciens/metabolism; Amino Acid Motifs; Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Mutation; Transcription Factors/chemistry; Transcription Factors/genetics; Transcription Factors/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:2000143: negative regulation of DNA-dependent transcription, initiation |
ECO:0000315: |
P |
When looking at Figure 2 it is evident that the more mutations in the irr gene that impact Irr protein function have a negative impact on its ability to repress a mbfA-lacZ reporter gene. According to the paper a double mutation will have a small defect but a triple mutation will cause a large defect in the repressor function. |
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See also
References
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