PMID:22579450

Citation	Auesukaree, C, Koedrith, P, Saenpayavai, P, Asvarak, T, Benjaphokee, S, Sugiyama, M, Kaneko, Y, Harashima, S and Boonchird, C (2012) Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits. J. Biosci. Bioeng. 114:144-9
Abstract	For industrial applications, fermentation of ethanol at high temperature offers advantages such as reduction in cooling costs, reduced risk of microbial contamination and higher efficiency of fermentation processes including saccharification and continuous ethanol stripping. Three thermotolerant Saccharomyces cerevisiae isolates (C3723, C3751 and C3867) from Thai fruits were capable of growing and producing 38 g/L ethanol up to 41°C. Based on genetic analyses, these isolates were prototrophic and homothallic, with dominant homothallic and thermotolerant phenotypes. After short-term (30 min) and long-term (12 h) exposure at 37°C, expression levels increased for the heat stress-response genes HSP26, SSA4, HSP82, and HSP104 encoding the heat shock proteins small HSP, HSP70, HSP90 and the HSP100 family, respectively. In isolates C3723 and C3867, expression was significantly higher than that in reference isolates W303 and TISTR5606 for TPS1 encoding trehalose-6-phosphate synthase, NTH1 encoding neutral trehalase and GSY1 encoding glycogen synthase. The results suggested that continuous high expression of heat stress-response genes was important for the long-term, heat stress tolerance of these thermotolerant isolates.
Links	PubMed Online version:10.1016/j.jbiosc.2012.03.012
Keywords	Ethanol/metabolism; Fermentation; Fruit/microbiology; Gene Expression Regulation, Fungal/genetics; Genes, Fungal; Glucosyltransferases/genetics; Heat-Shock Response/genetics; Hot Temperature; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/isolation & purification; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcriptome; Trehalase/genetics; Trehalose/biosynthesis; Trehalose/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:HSP82		GO:0009408: response to heat	ECO:0000314:		P		Figure 3 represents assays on the amount of the protein present after heat was applied. Expression was measured using RNeasy Mini Kit. Selected primers were seen in table 1	complete CACAO 8982
YEAST:HSP26		GO:0009408: response to heat	ECO:0000314:		P		Figure 3 represents assays on the amount of the protein present after heat was applied. Expression was measured using RNeasy Mini Kit. Selected primers were seen in table 1	complete CACAO 8981
YEAST:SGS1		GO:0009408: response to heat	ECO:0000314:		P		Figure 3 represents assays on the amount of the protein present after heat was applied. Expression was measured using RNeasy Mini Kit. Selected primers were seen in table 1	complete CACAO 8985
YEAST:TREA		GO:0009408: response to heat			P		Figure 3 represents assays on the amount of the protein present after heat was applied. Expression was measured using RNeasy Mini Kit. Selected primers were seen in table 1	Missing: evidence CACAO 8987
YEAST:GYS1		GO:0009408: response to heat			P		Figure 3 represents assays on the amount of the protein present after heat was applied. Expression was measured using RNeasy Mini Kit. Selected primers were seen in table 1	Missing: evidence CACAO 8988

References

See Help:References for how to manage references in GONUTS.