PMID:22495451

Citation	Cary, JW, Harris-Coward, PY, Ehrlich, KC, Moore, GG, Wei, Q and Bhatnagar, D Functional and phylogenetic analysis of the Aspergillus ochraceoroseus aflQ (ordA) gene ortholog. Mycologia 104:857-64
Abstract	Within the Aspergillus parasiticus and A. flavus aflatoxin (AF) biosynthetic gene cluster the aflQ (ordA) and aflP (omtA) genes encode respectively an oxidoreductase and methyltransferase. These genes are required for the final steps in the conversion of sterigmatocystin (ST) to aflatoxin B(1) (AFB(1)). Aspergillus nidulans harbors a gene cluster that produces ST, as the aflQ and aflP orthologs are either non-functional or absent in the genome. Aspergillus ochraceoroseus produces both AF and ST, and it harbors an AF/ST biosynthetic gene cluster that is organized much like the A. nidulans ST cluster. The A. ochraceoroseus cluster also does not contain aflQ or aflP orthologs. However the ability of A. ochraceoroseus to produce AF would indicate that functional aflQ and aflP orthologs are present within the genome. Utilizing degenerate primers based on conserved regions of the A. flavus aflQ gene and an A. nidulans gene demonstrating the highest level of homology to aflQ, a putative aflQ ortholog was PCR amplified from A. ochraceoroseus genomic DNA. The A. ochraceoroseus aflQ ortholog demonstrated 57% amino acid identity to A. flavus AflQ. Transformation of an O-methylsterigmatocystin (OMST)-accumulating A. parasiticus aflQ mutant with the putative A. ochraceoroseus aflQ gene restored AF production. Although the aflQ gene does not reside in the AF/ST cluster it appears to be regulated in a manner similar to other A. ochraceoroseus AF/ST cluster genes. Phylogenetic analysis of AflQ and AflQ-like proteins from a number of ST- and AF-producing Aspergilli indicates that A. ochraceoroseus might be ancestral to A. nidulans and A. flavus.
Links	PubMed Online version:10.3852/11-328
Keywords	Aflatoxins/genetics; Aflatoxins/metabolism; Aspergillus/classification; Aspergillus/genetics; Aspergillus/metabolism; Cloning, Molecular; Conserved Sequence; Culture Media/chemistry; DNA Primers/genetics; DNA, Fungal/genetics; Fungal Proteins/genetics; Fungal Proteins/metabolism; Gene Expression Regulation, Fungal; Genes, Fungal; Methyltransferases/genetics; Methyltransferases/metabolism; Multigene Family; Mycological Typing Techniques; Phylogeny; Polymerase Chain Reaction; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Species Specificity; Sterigmatocystin/analogs & derivatives; Sterigmatocystin/metabolism; Transformation, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ASPPA:ORDA	involved_in	GO:0045122: aflatoxin biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ASPPA:ORDA		GO:0045122: aflatoxin biosynthetic process	ECO:0000315:		P		Figure 2. Mutant phenotype produced no aflatoxin B1 or aflatoxin G1.	complete CACAO 8553

References

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