PMID:22448874

Citation	Smirnov, SV, Sokolov, PM, Kodera, T, Sugiyama, M, Hibi, M, Shimizu, S, Yokozeki, K and Ogawa, J (2012) A novel family of bacterial dioxygenases that catalyse the hydroxylation of free L-amino acids. FEMS Microbiol. Lett. 331:97-104
Abstract	L-isoleucine-4-hydroxylase (IDO) is a recently discovered member of the Pfam family PF10014 (the former DUF 2257 family) of uncharacterized conserved bacterial proteins. To uncover the range of biochemical activities carried out by PF10014 members, eight in silico-selected IDO homologues belonging to the PF10014 were cloned and expressed in Escherichia coli. L-methionine, L-leucine, L-isoleucine and L-threonine were found to be catalysed by the investigated enzymes, producing L-methionine sulfoxide, 4-hydroxyleucine, 4-hydroxyisoleucine and 4-hydroxythreonine, respectively. An investigation of enzyme kinetics suggested the existence of a novel subfamily of bacterial dioxygenases within the PF10014 family for which free L-amino acids could be accepted as in vivo substrates. A hypothesis regarding the physiological significance of hydroxylated l-amino acids is also discussed.
Links	PubMed Online version:10.1111/j.1574-6968.2012.02558.x
Keywords	Amino Acids/metabolism; Bacteria/classification; Bacteria/enzymology; Bacteria/genetics; Cloning, Molecular; Dioxygenases/classification; Dioxygenases/genetics; Dioxygenases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Hydroxylation; Isoleucine/metabolism; Kinetics; Leucine/metabolism; Methionine/metabolism; Substrate Specificity; Threonine/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
METFK:Q1GXZ5		GO:0051213: dioxygenase activity	ECO:0000314:		F		Table 1, Fig 2, Table 2	complete CACAO 5457
METFK:Q1GXZ5	enables	GO:0051213: dioxygenase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
GLUOX:Q5FQD2		GO:0051213: dioxygenase activity	ECO:0000314:		F		Table 1, Fig 2, Table 2	complete CACAO 5458
GLUOX:Q5FQD2	enables	GO:0051213: dioxygenase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
AGRVI:Q0GPY6		GO:0051213: dioxygenase activity	ECO:0000314:		F		Table 1, Fig 2, Table 2	complete CACAO 5459
AGRVI:Q0GPY6	enables	GO:0051213: dioxygenase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
BORPD:A9IE89		GO:0051213: dioxygenase activity	ECO:0000314:		F		Table 1, Fig 2, Table 2	complete
BACTU:IDO		GO:0051213: dioxygenase activity	ECO:0000314:		F		Table 1, Fig 2, Table 2	complete CACAO 5454
BACTU:IDO	enables	GO:0051213: dioxygenase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
PANAA:A0A0H3L116		GO:0051213: dioxygenase activity	ECO:0000314:		F		Table 1, Fig 2, Table 2	complete CACAO 5455

References

See Help:References for how to manage references in GONUTS.