PMID:22408076

Citation	Yin, LL and Xue, HW (2012) The MADS29 transcription factor regulates the degradation of the nucellus and the nucellar projection during rice seed development. Plant Cell 24:1049-65
Abstract	The MADS box transcription factors are critical regulators of rice (Oryza sativa) reproductive development. Here, we here report the functional characterization of a rice MADS box family member, MADS29, which is preferentially expressed in the nucellus and the nucellar projection. Suppressed expression of MADS29 resulted in abnormal seed development; the seeds were shrunken, displayed a low grain-filling rate and suppressed starch biosynthesis, and contained abnormal starch granules. Detailed analysis indicated that the abnormal seed development is due to defective programmed cell death (PCD) of the nucellus and nucellar projection, which was confirmed by a TUNEL assay and transcriptome analysis. Further studies showed that expression of MADS29 is induced by auxin and MADS29 protein binds directly to the putative promoter regions of genes that encode a Cys protease and nucleotide binding site-Leu-rich repeat proteins, thereby stimulating the PCD. This study identifies MADS29 as a key regulator of early rice seed development by regulating the PCD of maternal tissues. It provides informative clues to elucidate the regulatory mechanism of maternal tissue degradation after fertilization and to facilitate the studies of endosperm development and seed filling.
Links	PubMed PMC3336122 Online version:10.1105/tpc.111.094854
Keywords	Apoptosis; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Oryza sativa/genetics; Oryza sativa/growth & development; Plant Proteins/genetics; Plant Proteins/metabolism; Promoter Regions, Genetic; Seeds/genetics; Seeds/growth & development; Transcription Factors/genetics; Transcription Factors/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ORYSJ:MAD29	involved_in	GO:0048316: seed development	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ORYSJ:MAD29		GO:0048316: seed development	ECO:0000315:		P		Figure 4	complete CACAO 8218
ORYSJ:MAD29		GO:0019252: starch biosynthetic process	ECO:0000315:		P		Figure 3	complete CACAO 8219
ORYSJ:MAD29		GO:0043068: positive regulation of programmed cell death	ECO:0000315:		P		Figure 5	complete CACAO 8220
ORYSJ:MAD29		GO:0005634: nucleus	ECO:0000314:		C		Figure 6	complete CACAO 8221
ORYSJ:MAD29	involved_in	GO:0019252: starch biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ORYSJ:MAD29	involved_in	GO:0043068: positive regulation of programmed cell death	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ORYSJ:MAD29	part_of	GO:0005634: nucleus	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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