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PMID:22393062
| Citation |
Konishi, A, Arakawa, S, Yue, Z and Shimizu, S (2012) Involvement of Beclin 1 in engulfment of apoptotic cells. J. Biol. Chem. 287:13919-29 |
|---|---|
| Abstract |
Efficient apoptotic cell engulfment is important for both tissue homeostasis and immune response in mammals. In the present study, we report that Beclin 1 (a regulator of autophagy) is required for apoptotic cell engulfment. The engulfment process was largely abolished in Beclin 1 knock-out cells, and Beclin 1 knockdown significantly decreased apoptotic cell internalization in macrophage and fibroblast cell lines. Beclin 1 was recruited to the early phagocytic cup along with the generation of phosphatidylinositol 3-phosphate and Rac1, which regulates actin dynamics in lamellipodia. No lamellipodia were formed in Beclin 1 knock-out cells, and Beclin 1 knockdown completely inhibited the promotion of engulfment by ectopic expression of Rac1. Beclin 1 was co-immunoprecipitated with Rac1. These data indicate that Beclin 1 coordinates actin dynamics and membrane phospholipid synthesis to promote efficient apoptotic cell engulfment. |
| Links |
PubMed PMC3340198 Online version:10.1074/jbc.M112.348375 |
| Keywords |
Animals; Apoptosis; Apoptosis Regulatory Proteins/metabolism; Autophagy; Embryonic Stem Cells/cytology; Gene Library; Humans; Image Processing, Computer-Assisted; Jurkat Cells; Membrane Proteins/metabolism; Mice; Microscopy, Electron/methods; NIH 3T3 Cells; Neuropeptides/metabolism; Phagocytosis; Phosphatidylinositol 3-Kinases/metabolism; Plasmids/metabolism; rac GTP-Binding Proteins/metabolism; rac1 GTP-Binding Protein/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0043652: engulfment of apoptotic cell |
ECO:0000315: |
P |
According to Figure 2B, a knockdown in beclin 1 led to a significant increase in the engulfment of dead Jurkat cells compared to the wild type. |
complete | ||||
|
involved_in |
GO:0043652: engulfment of apoptotic cell |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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