GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:22359504
| Citation |
Dunham-Ems, SM, Caimano, MJ, Eggers, CH and Radolf, JD (2012) Borrelia burgdorferi requires the alternative sigma factor RpoS for dissemination within the vector during tick-to-mammal transmission. PLoS Pathog. 8:e1002532 |
|---|---|
| Abstract |
While the roles of rpoS(Bb) and RpoS-dependent genes have been studied extensively within the mammal, the contribution of the RpoS regulon to the tick-phase of the Borrelia burgdorferi enzootic cycle has not been examined. Herein, we demonstrate that RpoS-dependent gene expression is prerequisite for the transmission of spirochetes by feeding nymphs. RpoS-deficient organisms are confined to the midgut lumen where they transform into an unusual morphotype (round bodies) during the later stages of the blood meal. We show that round body formation is rapidly reversible, and in vitro appears to be attributable, in part, to reduced levels of Coenzyme A disulfide reductase, which among other functions, provides NAD+ for glycolysis. Our data suggest that spirochetes default to an RpoS-independent program for round body formation upon sensing that the energetics for transmission are unfavorable. |
| Links |
PubMed PMC3280991 Online version:10.1371/journal.ppat.1002532 |
| Keywords |
Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Borrelia burgdorferi/genetics; Borrelia burgdorferi/metabolism; Disease Vectors; Ixodes; Lyme Disease/genetics; Lyme Disease/transmission; Microscopy, Confocal; Microscopy, Electron, Transmission; Reverse Transcriptase Polymerase Chain Reaction; Sigma Factor/genetics; Sigma Factor/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0044008: dissemination or transmission of symbiont from host by vector |
ECO:0000315: |
P |
Figure 4. Images show the negative effects of a lack of RpoS has on spirochetes in the formation of mobile bodies and transmission of symbiont. |
complete | ||||
|
involved_in |
GO:0044008: dissemination or transmission of symbiont from host by vector |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
