PMID:22252319

Citation	Crisucci, EM and Arndt, KM (2012) Paf1 restricts Gcn4 occupancy and antisense transcription at the ARG1 promoter. Mol. Cell. Biol. 32:1150-63
Abstract	The conserved Paf1 complex negatively regulates the expression of numerous genes, yet the mechanisms by which it represses gene expression are not well understood. In this study, we use the ARG1 gene as a model to investigate the repressive functions of the Paf1 complex in Saccharomyces cerevisiae. Our results indicate that Paf1 mediates repression of the ARG1 gene independently of the gene-specific repressor, ArgR/Mcm1. Rather, by promoting histone H2B lysine 123 ubiquitylation, Paf1 represses the ARG1 gene by negatively affecting Gcn4 occupancy at the promoter. Consistent with this observation, Gcn5 and its acetylation sites on histone H3 are required for full ARG1 derepression in paf1Δ cells, and the repressive effect of Paf1 is largely maintained when the ARG1 promoter directs transcription of a heterologous coding region. Derepression of the ARG1 gene in paf1Δ cells is accompanied by small changes in nucleosome occupancy, although these changes are subtle in comparison to those that accompany gene activation through amino acid starvation. Additionally, conditions that stimulate ARG1 transcription, including PAF1 deletion, lead to increased antisense transcription across the ARG1 promoter. This promoter-associated antisense transcription positively correlates with ARG1 sense transcription. Finally, our results indicate that Paf1 represses other genes through mechanisms similar to those used at the ARG1 gene.
Links	PubMed PMC3295010 Online version:10.1128/MCB.06262-11
Keywords	Acetylation; Arginase/genetics; Basic-Leucine Zipper Transcription Factors/metabolism; Chromatin Immunoprecipitation; Gene Expression Regulation, Fungal; Histones/chemistry; Histones/metabolism; Nuclear Proteins/metabolism; Nucleosomes/metabolism; Promoter Regions, Genetic; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription, Genetic; Ubiquitination
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:PAF1		GO:0000122: negative regulation of transcription from RNA polymerase II promoter	ECO:0000315:		P		Figure 1 shows greater ARG1 expression caused Paf1 deletion. This is due to Paf1 mediated negative regulation of RNA polymerase II.	complete CACAO 11166

References

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