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PMID:22185468

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Citation

Srivastava, A, Ohm, RA, Oxiles, L, Brooks, F, Lawrence, CB, Grigoriev, IV and Cho, Y (2012) A zinc-finger-family transcription factor, AbVf19, is required for the induction of a gene subset important for virulence in Alternaria brassicicola. Mol. Plant Microbe Interact. 25:443-52

Abstract

Alternaria brassicicola is a successful saprophyte and necrotrophic plant pathogen with a broad host range within the family Brassicaceae. It produces secondary metabolites that marginally affect virulence. Cell wall-degrading enzymes (CDWE) have been considered important for pathogenesis but none of them individually have been identified as significant virulence factors in A. brassicicola. In this study, knockout mutants of a gene, AbVf19, were created and produced considerably smaller lesions than the wild type on inoculated host plants. The presence of tandem zinc-finger domains in the predicted amino acid sequence and nuclear localization of AbVf19-reporter protein suggested that it was a transcription factor. Gene expression comparisons using RNA-seq identified 74 genes being downregulated in the mutant during a late stage of infection. Among the 74 downregulated genes, 28 were putative CWDE genes. These were hydrolytic enzyme genes that composed a small fraction of genes within each family of cellulases, pectinases, cutinases, and proteinases. The mutants grew slower than the wild type on an axenic medium with pectin as a major carbon source. This study demonstrated the existence and the importance of a transcription factor that regulates a suite of genes that are important for decomposing and utilizing plant material during the late stage of plant infection.

Links

PubMed Online version:10.1094/MPMI-10-11-0275

Keywords

Alternaria/genetics; Alternaria/metabolism; Alternaria/pathogenicity; Arabidopsis/genetics; Arabidopsis/microbiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Brassica/microbiology; Cytochrome P-450 Enzyme System/genetics; Cytochrome P-450 Enzyme System/metabolism; Fungal Proteins/genetics; Fungal Proteins/metabolism; Gene Expression Regulation, Fungal/physiology; Gene Expression Regulation, Plant/physiology; Molecular Sequence Data; Mutation; Plant Diseases/microbiology; Spores, Fungal; Transcription Factors/genetics; Transcription Factors/metabolism; Virulence

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ALTBR:K4H1X2

GO:0005634: nucleus

ECO:0000314:

C

Figure 5

complete
CACAO 8273

ALTBR:K4H1X2

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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