PMID:22155153

Citation	Evanko, SP, Potter-Perigo, S, Bollyky, PL, Nepom, GT and Wight, TN (2012) Hyaluronan and versican in the control of human T-lymphocyte adhesion and migration. Matrix Biol. 31:90-100
Abstract	The ability of lymphocytes to migrate freely through connective tissues is vital to efficient immune function. How the extracellular matrix (ECM) may affect T-cell adhesion and migration is not well understood. We have examined the adhesion and migration of activated human T-lymphocytes on ECM made by fibroblast-like synoviocytes and lung fibroblasts. These cells were minimally interactive until treated with a viral mimetic, Poly I:C. This treatment promoted myofibroblast formation and engendered a higher-order structured ECM, rich in versican and hyaluronan, to which T-cells avidly adhered in a hyaluronidase-sensitive manner. This Poly I:C-induced matrix impeded T-cell spreading and migration on and through synoviocyte monolayers, while hyaluronidase treatment or adding versican antibody during matrix formation reversed the effect on T-cell migration. Hyaluronidase also reversed the spread myofibroblast morphology. These data suggest that the viscous hyaluronan- and versican-rich matrix binds and constrains T-lymphocytes. Using purified matrix components and solid state matrices of defined composition, we uncovered a role for versican in modulating hyaluronan-T-cell interactions. Versican prevented T-cell binding to soluble hyaluronan, as well as the amoeboid shape change on hyaluronan-coated dishes and T-cell penetration of collagen gels. Together, these data suggest that hyaluronan and versican play a role in T-cell trafficking and function in inflamed tissues.
Links	PubMed PMC3288568 Online version:10.1016/j.matbio.2011.10.004
Keywords	Antigens, CD44/metabolism; CD4-Positive T-Lymphocytes/drug effects; CD4-Positive T-Lymphocytes/metabolism; CD4-Positive T-Lymphocytes/physiology; Cell Adhesion; Cell Movement; Cells, Cultured; Collagen/metabolism; Extracellular Matrix/metabolism; Fibroblasts/drug effects; Fibroblasts/metabolism; Fibroblasts/physiology; Fluorescein-5-isothiocyanate/metabolism; Humans; Hyaluronic Acid/metabolism; Hyaluronic Acid/pharmacology; Hyaluronoglucosaminidase/metabolism; Inflammation/metabolism; Lung/cytology; Lymphocyte Activation; Poly I-C/pharmacology; Protein Binding; Time-Lapse Imaging/methods; Versicans/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BOVIN:CSPG2		GO:0031012: extracellular matrix	ECO:0000314:		C		Fig. 2	complete

References

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