PMID:21917855

Citation	Asagoshi, K, Lehmann, W, Braithwaite, EK, Santana-Santos, L, Prasad, R, Freedman, JH, Van Houten, B and Wilson, SH (2012) Single-nucleotide base excision repair DNA polymerase activity in C. elegans in the absence of DNA polymerase β. Nucleic Acids Res. 40:670-81
Abstract	The base excision DNA repair (BER) pathway known to occur in Caenorhabditis elegans has not been well characterized. Even less is known about the DNA polymerase (pol) requirement for the gap-filling step during BER. We now report on characterization of in vitro uracil-DNA initiated BER in C. elegans. The results revealed single-nucleotide (SN) gap-filling DNA polymerase activity and complete BER. The gap-filling polymerase activity was not due to a DNA polymerase β (pol β) homolog, or to another X-family polymerase, since computer-based sequence analyses of the C. elegans genome failed to show a match for a pol β-like gene or other X-family polymerases. Activity gel analysis confirmed the absence of pol β in the C. elegans extract. BER gap-filling polymerase activity was partially inhibited by both dideoxynucleotide and aphidicolin. The results are consistent with a combination of both replicative polymerase(s) and lesion bypass/BER polymerase pol θ contributing to the BER gap-filling synthesis. Involvement of pol θ was confirmed in experiments with extract from pol θ null animals. The presence of the SN BER in C. elegans is supported by these results, despite the absence of a pol β-like enzyme or other X-family polymerase.
Links	PubMed PMC3258131 Online version:10.1093/nar/gkr727
Keywords	Animals; Aphidicolin/pharmacology; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/growth & development; DNA/biosynthesis; DNA Polymerase beta/genetics; DNA Repair; DNA-Directed DNA Polymerase/genetics; DNA-Directed DNA Polymerase/metabolism; Gene Deletion; Genomics; Open Reading Frames
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CAEEL:DPOLQ		GO:0006287: base-excision repair, gap-filling	ECO:0000315:		P		Figure 5: BER activity from the polq-1 mutation extract lower compared to the BER activity in the wild-type extract prepared in parallel	complete CACAO 6839
CAEEL:DPOLQ	involved_in	GO:0006287: base-excision repair, gap-filling	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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