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PMID:21900491
| Citation |
Bunnell, TM, Burbach, BJ, Shimizu, Y and Ervasti, JM (2011) β-Actin specifically controls cell growth, migration, and the G-actin pool. Mol. Biol. Cell 22:4047-58 |
|---|---|
| Abstract |
Ubiquitously expressed β-actin and γ-actin isoforms play critical roles in most cellular processes; however, their unique contributions are not well understood. We generated whole-body β-actin-knockout (Actb(-/-)) mice and demonstrated that β-actin is required for early embryonic development. Lethality of Actb(-/-) embryos correlated with severe growth impairment and migration defects in β-actin-knockout primary mouse embryonic fibroblasts (MEFs) that were not observed in γ-actin-null MEFs. Migration defects were associated with reduced membrane protrusion dynamics and increased focal adhesions. We also identified migration defects upon conditional ablation of β-actin in highly motile T cells. Of great interest, ablation of β-actin altered the ratio of globular actin (G-actin) to filamentous actin in MEFs, with corresponding changes in expression of genes that regulate the cell cycle and motility. These data support an essential role for β-actin in regulating cell migration and gene expression through control of the cellular G-actin pool. |
| Links |
PubMed PMC3204067 Online version:10.1091/mbc.E11-06-0582 |
| Keywords |
Actins/deficiency; Actins/genetics; Actins/metabolism; Animals; Brain/metabolism; CD4-Positive T-Lymphocytes/metabolism; CD4-Positive T-Lymphocytes/physiology; Cell Movement; Cell Proliferation; Cells, Cultured; Female; Fibroblasts; Gene Expression Profiling; Gene Expression Regulation; Gene Knockout Techniques; Heterozygote; Kaplan-Meier Estimate; Kidney/metabolism; Liver/metabolism; Lung/metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Organ Specificity; Primary Cell Culture |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016049: cell growth |
ECO:0000314: |
P |
Figure 3 shows severe growth deficiency in the absence of β-actin. |
complete | ||||
See also
References
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