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PMID:21895628
| Citation |
Oddi, S, Dainese, E, Sandiford, S, Fezza, F, Lanuti, M, Chiurchiù, V, Totaro, A, Catanzaro, G, Barcaroli, D, De Laurenzi, V, Centonze, D, Mukhopadhyay, S, Selent, J, Howlett, AC and Maccarrone, M (2012) Effects of palmitoylation of Cys(415) in helix 8 of the CB(1) cannabinoid receptor on membrane localization and signalling. Br. J. Pharmacol. 165:2635-51 |
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| Abstract |
The CB(1) cannabinoid receptor is regulated by its association with membrane microdomains such as lipid rafts. Here, we investigated the role of palmitoylation of the CB(1) receptor by analysing the functional consequences of site-specific mutation of Cys(415) , the likely site of palmitoylation at the end of helix 8, in terms of membrane association, raft targeting and signalling. |
| Links |
PubMed PMC3423250 Online version:10.1111/j.1476-5381.2011.01658.x |
| Keywords |
Animals; Cell Line; Cell Membrane/metabolism; Cysteine/chemistry; Cysteine/genetics; Green Fluorescent Proteins/metabolism; HEK293 Cells; Humans; Lipoylation; Mutation; Prosencephalon/metabolism; Rats; Receptor, Cannabinoid, CB1/chemistry; Receptor, Cannabinoid, CB1/genetics; Receptor, Cannabinoid, CB1/metabolism; Signal Transduction |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0045121: membrane raft |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0045121: membrane raft |
ECO:0000314: |
C |
Figure 6c: Alignment was observed between CTBs (lipid rafts)marked with red florescent proteins and the CB1 (cannabinoid receptor 1) marked with GFP. This suggests that CB1 is a membrane bound protein associated with lipid rafts. |
complete | ||||
See also
References
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