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PMID:21889053
| Citation |
Hadži-Tašković Šukalović, V, Vuletić, M, Marković, K and Vučinić, Z (2011) Cell wall-associated malate dehydrogenase activity from maize roots. Plant Sci. 181:465-70 |
|---|---|
| Abstract |
Isolated cell walls from maize (Zea mays L.) roots exhibited ionically and covalently bound NAD-specific malate dehydrogenase activity. The enzyme catalyses a rapid reduction of oxaloacetate and much slower oxidation of malate. The kinetic and regulatory properties of the cell wall enzyme solubilized with 1M NaCl were different from those published for soluble, mitochondrial or plasma membrane malate dehydrogenase with respect to their ATP, Pi, and pH dependence. Isoelectric focusing of ionically-bound proteins and specific staining for malate dehydrogenase revealed characteristic isoforms present in cell wall isolate, different from those present in plasma membranes and crude homogenate. Much greater activity of cell wall-associated malate dehydrogenase was detected in the intensively growing lateral roots compared to primary root with decreased growth rates. Presence of Zn(2+) and Cu(2+) in the assay medium inhibited the activity of the wall-associated malate dehydrogenase. Exposure of maize plants to excess concentrations of Zn(2+) and Cu(2+) in the hydroponic solution inhibited lateral root growth, decreased malate dehydrogenase activity and changed isoform profiles. The results presented show that cell wall malate dehydrogenase is truly a wall-bound enzyme, and not an artefact of cytoplasmic contamination, involved in the developmental processes, and detoxification of heavy metals. |
| Links |
PubMed Online version:10.1016/j.plantsci.2011.07.007 |
| Keywords |
Cell Wall/drug effects; Cell Wall/enzymology; Copper/pharmacology; Hydrogen-Ion Concentration/drug effects; Isoelectric Focusing; Isoenzymes/isolation & purification; Isoenzymes/metabolism; Kinetics; Malate Dehydrogenase/isolation & purification; Malate Dehydrogenase/metabolism; Malates/metabolism; Oxaloacetates/metabolism; Oxidation-Reduction/drug effects; Plant Roots/cytology; Plant Roots/drug effects; Plant Roots/enzymology; Protein Binding/drug effects; Solubility/drug effects; Subcellular Fractions/drug effects; Subcellular Fractions/enzymology; Zea mays/cytology; Zea mays/drug effects; Zea mays/enzymology; Zea mays/growth & development; Zinc/pharmacology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0030060: L-malate dehydrogenase activity |
ECO:0000314: |
F |
See Figure 1 for L-malate dehydrogenase activity |
complete | ||||
| GO:0016615: malate dehydrogenase activity |
ECO:0000314: |
F |
See Figure 1 for malate oxidation activity. |
complete | ||||
| GO:0009505: plant-type cell wall |
ECO:0000314: |
C |
Figure 1 shows the Malate Dehydrogenase activity in the cell walls of the maize root. |
complete | ||||
| GO:0044459: plasma membrane part |
ECO:0000314: |
C |
See Figure 2 for Malate Dehydrogenase activity presence in plasma membrane. |
complete | ||||
| GO:1901001: negative regulation of response to salt stress |
ECO:0000314: |
P |
Table 3 shows the inhibitory effects on Malate Dehydrogenase by CuCl2 and ZnCl2 salts with an addition of oxaloacetate. |
complete | ||||
|
enables |
GO:0016615: malate dehydrogenase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0009505: plant-type cell wall |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005886: plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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