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PMID:21816350
| Citation |
Salje, J, van den Ent, F, de Boer, P and Löwe, J (2011) Direct membrane binding by bacterial actin MreB. Mol. Cell 43:478-87 |
|---|---|
| Abstract |
Bacterial actin MreB is one of the key components of the bacterial cytoskeleton. It assembles into short filaments that lie just underneath the membrane and organize the cell wall synthesis machinery. Here we show that MreB from both T. maritima and E. coli binds directly to cell membranes. This function is essential for cell shape determination in E. coli and is proposed to be a general property of many, if not all, MreBs. We demonstrate that membrane binding is mediated by a membrane insertion loop in TmMreB and by an N-terminal amphipathic helix in EcMreB and show that purified TmMreB assembles into double filaments on a membrane surface that can induce curvature. This, the first example of a membrane-binding actin filament, prompts a fundamental rethink of the structure and dynamics of MreB filaments within cells. |
| Links |
PubMed PMC3163269 Online version:10.1016/j.molcel.2011.07.008 |
| Keywords |
Actin Cytoskeleton/chemistry; Actin Cytoskeleton/metabolism; Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Bacterial Proteins/physiology; Cell Membrane/metabolism; Cell Membrane/ultrastructure; Cytoskeletal Proteins/chemistry; Cytoskeletal Proteins/metabolism; Cytoskeletal Proteins/physiology; Escherichia coli/metabolism; Escherichia coli/ultrastructure; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/physiology; Green Fluorescent Proteins/analysis; Models, Molecular; Molecular Sequence Data; Protein Structure, Tertiary; Sequence Alignment; Thermotoga maritima/metabolism; Thermotoga maritima/ultrastructure |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0031226: intrinsic component of plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
Notes
See also
References
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