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PMID:21763308
| Citation |
Kim, SM, Bae, J, Cho, IH, Choi, KY, Park, YJ, Ryu, JH, Chun, JS and Song, WK (2011) Control of growth cone motility and neurite outgrowth by SPIN90. Exp. Cell Res. 317:2276-87 |
|---|---|
| Abstract |
SPIN90 is an F-actin binding protein thought to play important roles in regulating cytoskeletal dynamics. It is known that SPIN90 is expressed during the early stages of neuronal development, but details of its localization and function in growth cones have not been fully investigated. Our immunocytochemical data show that SPIN90 is enriched throughout growth cones and neuronal shafts in young hippocampal neurons. We also found that its localization correlates with and depends upon the presence of F-actin. Detailed observation of primary cultures of hippocampal neurons revealed that SPIN90 knockout reduces both growth cone areas and in the numbers of filopodia, as compared to wild-type neurons. In addition, total neurite length, the combined lengths of the longest (axonal) and shorter (dendritic) neurites, was smaller in SPIN90 knockout neurons than wild-type neurons. Finally, Cdc42 activity was down-regulated in SPIN90 knockout neurons. Taken together, our findings suggest that SPIN90 plays critical roles in controlling growth cone dynamics and neurite outgrowth. |
| Links |
PubMed Online version:10.1016/j.yexcr.2011.06.018 |
| Keywords |
Actin Cytoskeleton/drug effects; Actin Cytoskeleton/metabolism; Actins/metabolism; Adaptor Proteins, Signal Transducing/genetics; Adaptor Proteins, Signal Transducing/metabolism; Animals; Bicyclo Compounds, Heterocyclic/pharmacology; Cell Differentiation/physiology; Cells, Cultured; Cytochalasin D/pharmacology; Embryo, Mammalian/cytology; Female; Growth Cones/pathology; Growth Cones/physiology; Hippocampus/cytology; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Microtubules/metabolism; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurites/pathology; Neurites/physiology; Neurons/cytology; Neurons/drug effects; Neurons/metabolism; Neurons/pathology; Pseudopodia/metabolism; Pseudopodia/pathology; Rats; Rats, Inbred Strains; Thiazolidines/pharmacology; Tubulin/metabolism; Vesicle-Associated Membrane Protein 2/metabolism; cdc42 GTP-Binding Protein/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0040008: regulation of growth |
ECO:0000315: |
P |
Figure 4 - SPIN90 affects cytoskeleton organization by "regulating growth cone morphology" |
complete | ||||
| GO:0010976: positive regulation of neuron projection development |
ECO:0000315: |
P |
Figure 6. When SPIN90 was knocked out, neurite outgrowth was inhibited. This was shown by comparing the length of the longest neurite and the length of shorter neurites of wild-type and of SPIN90 knocked-out hippocampal neurons. |
complete | ||||
|
involved_in |
GO:0010976: positive regulation of neuron projection development |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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