PMID:21576394

Citation	Gudi, R, Zou, C, Li, J and Gao, Q (2011) Centrobin-tubulin interaction is required for centriole elongation and stability. J. Cell Biol. 193:711-25
Abstract	Centrobin is a daughter centriole protein that is essential for centrosome duplication. However, the molecular mechanism by which centrobin functions during centriole duplication remains undefined. In this study, we show that centrobin interacts with tubulin directly, and centrobin-tubulin interaction is pivotal for the function of centrobin during centriole duplication. We found that centrobin is recruited to the centriole biogenesis site via its interaction with tubulins during the early stage of centriole biogenesis, and its recruitment is dependent on hSAS-6 but not centrosomal P4.1-associated protein (CPAP) and CP110. The function of centrobin is also required for the elongation of centrioles, which is likely mediated by its interaction with tubulin. Furthermore, disruption of centrobin-tubulin interaction led to destabilization of existing centrioles and the preformed procentriole-like structures induced by CPAP expression, indicating that centrobin-tubulin interaction is critical for the stability of centrioles. Together, our study demonstrates that centrobin facilitates the elongation and stability of centrioles via its interaction with tubulins.
Links	PubMed PMC3166857 Online version:10.1083/jcb.201006135
Keywords	Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Centrioles/drug effects; Centrioles/metabolism; Fluorescent Antibody Technique; HEK293 Cells; HeLa Cells; Humans; Hydroxyurea/pharmacology; Microtubule-Associated Proteins/metabolism; Phosphoproteins/metabolism; Protein Binding; Protein Interaction Domains and Motifs; Protein Interaction Mapping; Protein Multimerization; RNA Interference; Transfection; Tubulin/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:CNTRB		GO:0007099: positive regulation of centriole replication	ECO:0000315:		P		Figure 3	complete CACAO 6966
HUMAN:CNTRB		GO:0005813: protein localization to centrosome	ECO:0000315:		P		Figure 5	complete CACAO 6969
HUMAN:CNTRB	involved_in	GO:0007099: centriole replication	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:CNTRB	part_of	GO:0005813: centrosome	ECO:0000315: mutant phenotype evidence used in manual assertion		C		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.