PMID:21479242

Citation	Suh, YH, Yoshimoto-Furusawa, A, Weih, KA, Tessarollo, L, Roche, KW, Mackem, S and Roche, PA (2011) Deletion of SNAP-23 results in pre-implantation embryonic lethality in mice. PLoS ONE 6:e18444
Abstract	SNARE-mediated membrane fusion is a pivotal event for a wide-variety of biological processes. SNAP-25, a neuron-specific SNARE protein, has been well-characterized and mouse embryos lacking Snap25 are viable. However, the phenotype of mice lacking SNAP-23, the ubiquitously expressed SNAP-25 homolog, remains unknown. To reveal the importance of SNAP-23 function in mouse development, we generated Snap23-null mice by homologous recombination. We were unable to obtain newborn SNAP-23-deficient mice, and analysis of pre-implantation embryos from Snap23(Δ/wt) matings revealed that Snap23-null blastocysts were dying prior to implantation at embryonic day E3.5. Thus these data reveal a critical role for SNAP-23 during embryogenesis.
Links	PubMed PMC3066230 Online version:10.1371/journal.pone.0018444
Keywords	Alleles; Animals; Blastocyst/cytology; Blastocyst/metabolism; Breeding; Cell Death; Embryo Implantation; Embryo Loss/metabolism; Embryo Loss/pathology; Female; Gene Deletion; Gene Targeting; Heterozygote; Mice; Mice, Inbred C57BL; Pregnancy; Qb-SNARE Proteins/deficiency; Qb-SNARE Proteins/metabolism; Qc-SNARE Proteins/deficiency; Qc-SNARE Proteins/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:SNP23		GO:0007566: embryo implantation	ECO:0000315:		P		Fig 4 and Table 2. Blastocyst stage embryos were recovered by flushing from the uterus of pregnant mice at day E3.5. 5 among a total of 27 blastocysts isolated using this procedure appeared grossly abnormal and had not expanded properly, suggesting that these blastocysts were dying, and unlike the normal blastocysts, they failed to develop any further after 24 hrs of culture. Each of five small, degenerating blastocysts showed a Snap23Δ/Δ genotype, whereas the morphologically normal embryos included 11 SNAP23Δ/wt heterozygotes and 11 SNAP23wt/wt wild-type embryos.	complete
MOUSE:SNP23		GO:0043009: chordate embryonic development	ECO:0000315:		P		To generate Snap23-deficient mice, Snap23Δ/wt mice were mated with each other. Snap23Δ/Δ pups were never obtained from adult heterozygous matings after more than 50 live births. Genotyping confirmed that approximately 2/3 of these offspring were Snap23Δ/wt and 1/3 were Snap23wt/wt, demonstrating that deletion of Snap23 leads to embryonic lethality.	complete

References

See Help:References for how to manage references in GONUTS.