PMID:21346197

Citation	Helfand, BT, Mendez, MG, Murthy, SN, Shumaker, DK, Grin, B, Mahammad, S, Aebi, U, Wedig, T, Wu, YI, Hahn, KM, Inagaki, M, Herrmann, H and Goldman, RD (2011) Vimentin organization modulates the formation of lamellipodia. Mol. Biol. Cell 22:1274-89
Abstract	Vimentin intermediate filaments (VIF) extend throughout the rear and perinuclear regions of migrating fibroblasts, but only nonfilamentous vimentin particles are present in lamellipodial regions. In contrast, VIF networks extend to the entire cell periphery in serum-starved or nonmotile fibroblasts. Upon serum addition or activation of Rac1, VIF are rapidly phosphorylated at Ser-38, a p21-activated kinase phosphorylation site. This phosphorylation of vimentin is coincident with VIF disassembly at and retraction from the cell surface where lamellipodia form. Furthermore, local induction of photoactivatable Rac1 or the microinjection of a vimentin mimetic peptide (2B2) disassemble VIF at sites where lamellipodia subsequently form. When vimentin organization is disrupted by a dominant-negative mutant or by silencing, there is a loss of polarity, as evidenced by the formation of lamellipodia encircling the entire cell, as well as reduced cell motility. These findings demonstrate an antagonistic relationship between VIF and the formation of lamellipodia.
Links	PubMed PMC3078081 Online version:10.1091/mbc.E10-08-0699
Keywords	Animals; Cell Movement; Cell Polarity; Escherichia coli; Gene Expression; Gene Silencing; Humans; Intermediate Filaments/metabolism; Mice; Mice, Knockout; Microinjections; NIH 3T3 Cells; Neuropeptides/genetics; Neuropeptides/metabolism; Peptide Fragments/genetics; Peptide Fragments/metabolism; Phosphorylation; Pseudopodia/genetics; Pseudopodia/metabolism; RNA, Small Interfering/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Serine/metabolism; Serum/metabolism; Vimentin/genetics; Vimentin/metabolism; p21-Activated Kinases/genetics; p21-Activated Kinases/metabolism; rac GTP-Binding Proteins/genetics; rac GTP-Binding Proteins/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:VIME		GO:0010762: regulation of fibroblast migration	ECO:0000315:		P		Vimentin involvement in lamellipodia formation was inferred from silencing experiments.	complete CACAO 7171

References

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