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PMID:21283621
Citation |
Chansard, M, Wang, J, Tran, HC, Neumayer, G, Shim, SY, Park, YU, Belzil, C, Le, HT, Park, SK and Nguyen, MD (2011) The cytoskeletal protein Ndel1 regulates dynamin 2 GTPase activity. PLoS ONE 6:e14583 |
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Abstract |
Cytoskeleton dynamics, membranes trafficking and positioning are essential for the proper functioning of any mammalian cell. The identification of the molecules and mechanisms that allow these cellular processes to interface is vital for understanding cell behaviors. Ndel1, the mammalian homolog of the Aspergillus nidulans NudE, organizes the cytoskeleton and regulates molecular motors, thereby impacting on the positioning of membranes. Hypothetically, Ndel1 can act in concert with enzymes controlling membrane trafficking (vesicle-mediated transport) per se, but this idea has never been investigated. We now report that a pool of Ndel1 associates directly with Dynamin 2 (Dyn2), a large cytosolic GTPase involved in the trafficking of the AMPA receptor subunit GluR1. In vitro, Ndel1 enhances Dyn2 GTPase activity in its unassembled and assembled forms, without promoting oligomerization of the enzyme. In cells, gain and loss of function of Ndel1 recapitulate the effects of overexpression of Dyn2 and Dyn2 dominant negative with reduced GTPase activity on the intracellular localization of GluR1, respectively, without affecting the stability of microtubules. Together, these results indicate that Ndel1 regulates Dyn2 GTPase activity and impacts GluR1-containing membranes distribution in a manner reminiscent of Dyn2. |
Links |
PubMed PMC3026782 Online version:10.1371/journal.pone.0014583 |
Keywords |
Biological Transport; Carrier Proteins/physiology; Cell Line; Cell Membrane; Cytoskeletal Proteins/metabolism; Dynamin II/metabolism; GTP Phosphohydrolases/metabolism; Humans; Receptors, AMPA/metabolism |
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Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0043547: positive regulation of GTPase activity |
ECO:0000315: |
P |
Fig. 3 |
complete | ||||
GO:0033157: regulation of intracellular protein transport |
ECO:0000315: |
P |
Fig. 5 (especially 5C) |
complete | ||||
involved_in |
GO:0033157: regulation of intracellular protein transport |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
involved_in |
GO:0043547: positive regulation of GTPase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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