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PMID:21239581
| Citation |
Rahn-Lee, L, Merrikh, H, Grossman, AD and Losick, R (2011) The sporulation protein SirA inhibits the binding of DnaA to the origin of replication by contacting a patch of clustered amino acids. J. Bacteriol. 193:1302-7 |
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| Abstract |
Bacteria regulate the frequency and timing of DNA replication initiation by controlling the activity of the replication initiator protein DnaA. SirA is a recently discovered regulator of DnaA in Bacillus subtilis whose synthesis is turned on at the start of sporulation. Here, we demonstrate that SirA contacts DnaA at a patch of 3 residues located on the surface of domain I of the replication initiator protein, corresponding to the binding site used by two unrelated regulators of DnaA found in other bacteria. We show that the interaction of SirA with domain I inhibits the ability of DnaA to bind to the origin of replication. DnaA mutants containing amino acid substitutions of the 3 residues are functional in replication initiation but are immune to inhibition by SirA. |
| Links |
PubMed PMC3067619 Online version:10.1128/JB.01390-10 |
| Keywords |
Amino Acid Substitution/genetics; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Binding Sites; DNA Replication; DNA, Bacterial/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Protein Binding; Protein Interaction Domains and Motifs; Protein Interaction Mapping; Replication Origin |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008156: negative regulation of DNA replication |
ECO:0000315: |
P |
Fig. 5 indicates that SirA reduces the amount of wild-type but not mutant DnaA bound at the origin of replication. Therefore this results indicate that interaction of SirA contacts DnaA at a patch of 3 residues located on the surface of domain I of the replication initiator protein and inhibits the ability of DNA to bind to the origin of replication. |
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