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PMID:21215257
| Citation |
Zhang, L, Li, J, Jiang, Z, Sun, L, Mei, X, Yong, B and Zhang, L (2011) Inhibition of aquaporin-1 expression by RNAi protects against aristolochic acid I-induced apoptosis in human proximal tubular epithelial (HK-2) cells. Biochem. Biophys. Res. Commun. 405:68-73 |
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| Abstract |
The aim of this study was to investigate the protective effect of inhibition of aquaporin-1 (AQP1) expression against aristolochic acid I (AA-I)-induced apoptosis. HK-2 cells impaired by AA-I were used in this study as the cell model of aristolochic acid nephropathy. Apoptosis was studied by different methods, including 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assays, flow cytometry, and caspase 3 activity assays. We compared AA-I-mediated apoptosis in HK-2 cells with or without knockdown of AQP1 expression by RNA interference. MTT assays showed that AA-I inhibited the viability of HK-2 cells in a time- and concentration-dependent manner. Apoptosis was evidenced by the results of the Annexin V/propidium iodide assay and the occurrence of a sub-G1 peak in cell-cycle analysis. The activity of caspase 3 was found to have been increased by AA-I in a concentration-dependent manner. However, AQP1 RNA interference provided protection against injury in cells treated with AA-I (40 μM) for 24 h and attenuated the number of apoptotic cells. These results suggested that AQP1 plays an important role in AA-I-induced apoptosis and that inhibition of AQP1 expression may protect HK-2 cells from AA-I-induced apoptotic damage. |
| Links |
PubMed Online version:10.1016/j.bbrc.2010.12.128 |
| Keywords |
Apoptosis/genetics; Aquaporin 1/genetics; Aristolochic Acids/toxicity; Cell Line; Epithelial Cells/drug effects; Epithelial Cells/pathology; Humans; Kidney Diseases/chemically induced; Kidney Diseases/pathology; Kidney Tubules, Proximal/drug effects; Kidney Tubules, Proximal/pathology; Models, Biological; RNA Interference |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0043154: negative regulation of cysteine-type endopeptidase activity involved in apoptotic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
occurs_in:(CL:0002306) |
Seeded From UniProt |
complete | ||
See also
References
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