PMID:21177654

Citation	Anno, YN, Myslinski, E, Ngondo-Mbongo, RP, Krol, A, Poch, O, Lecompte, O and Carbon, P (2011) Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription. Nucleic Acids Res. 39:3116-27
Abstract	In the human genome, ∼ 10% of the genes are arranged head to head so that their transcription start sites reside within <1 kbp on opposite strands. In this configuration, a bidirectional promoter generally drives expression of the two genes. How bidirectional expression is performed from these particular promoters constitutes a puzzling question. Here, by a combination of in silico and biochemical approaches, we demonstrate that hStaf/ZNF143 is involved in controlling expression from a subset of divergent gene pairs. The binding sites for hStaf/ZNF143 (SBS) are overrepresented in bidirectional versus unidirectional promoters. Chromatin immunoprecipitation assays with a significant set of bidirectional promoters containing putative SBS revealed that 93% of them are associated with hStaf/ZNF143. Expression of dual reporter genes directed by bidirectional promoters are dependent on the SBS integrity and requires hStaf/ZNF143. Furthermore, in some cases, functional SBS are located in bidirectional promoters of gene pairs encoding a noncoding RNA and a protein gene. Remarkably, hStaf/ZNF143 per se exhibits an inherently bidirectional transcription activity, and together our data provide the demonstration that hStaf/ZNF143 is indeed a transcription factor controlling the expression of divergent protein-protein and protein-non-coding RNA gene pairs.
Links	PubMed PMC3082894 Online version:10.1093/nar/gkq1301
Keywords	Binding Sites; DNA/chemistry; Down-Regulation; Gene Knockdown Techniques; Genome, Human; HeLa Cells; Humans; Promoter Regions, Genetic; Proteins/genetics; RNA, Small Nuclear/genetics; RNA, Small Nuclear/metabolism; Sequence Analysis, DNA; Trans-Activators/genetics; Trans-Activators/metabolism; Trans-Activators/physiology; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:ZN143		GO:0045449 : regulation of transcription	ECO:0000314:		P		See Figure 5, pg. 10.	complete
HUMAN:ZN143		GO:0016563: transcription activator activity	ECO:0000314:		F		See Figure 2A, 2C, 2D, and 2E. See Figure 5.	complete

References

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