PMID:21048011

Citation	Gu, J, Xu, W, Lei, L, Huang, J, Feng, X, Sun, C, Du, C, Zuo, J, Li, Y, Du, T, Li, L and Han, W (2011) LysGH15, a novel bacteriophage lysin, protects a murine bacteremia model efficiently against lethal methicillin-resistant Staphylococcus aureus infection. J. Clin. Microbiol. 49:111-7
Abstract	Phage-coded lysin is an enzyme that destroys the cell walls of bacteria. Phage lysin could be an alternative to conventional antibiotic therapy against pathogens that are resistant to multiple antibiotics. In this study, a novel staphylococcal phage, GH15, was isolated, and the endogenous lytic enzyme (LysGH15) was expressed and purified. The lysin LysGH15 displayed a broad lytic spectrum; in vitro treatment killed a number of Staphylococcus aureus strains rapidly and completely, including methicillin-resistant S. aureus (MRSA). In animal experiments, a single intraperitoneal injection of LysGH15 (50 μg) administered 1 h after MRSA injections at double the minimum lethal dose was sufficient to protect mice (P < 0.01). Bacteremia in unprotected mice reached colony counts of about 10(7) CFU/ml within 3.5 h after challenge, whereas the mean colony count in lysin-protected mice was less than 10(4) CFU/ml (and ultimately became undetectable). These results indicate that LysGH15 can kill S. aureus in vitro and can protect mice efficiently from bacteremia in vivo. The phage lysin LysGH15 might be an alternative treatment strategy for infections caused by MRSA.
Links	PubMed PMC3020447 Online version:10.1128/JCM.01144-10
Keywords	Animals; Bacteremia/microbiology; Bacteremia/prevention & control; DNA, Viral/chemistry; DNA, Viral/genetics; Disease Models, Animal; Female; Humans; Methicillin-Resistant Staphylococcus aureus/drug effects; Mice; Mice, Inbred BALB C; Microbial Viability/drug effects; Molecular Sequence Data; Mucoproteins/administration & dosage; Mucoproteins/genetics; Mucoproteins/isolation & purification; Sequence Analysis, DNA; Staphylococcal Infections/microbiology; Staphylococcal Infections/prevention & control; Staphylococcus Phages/enzymology; Staphylococcus Phages/isolation & purification; Survival Analysis; Treatment Outcome; Viral Proteins/administration & dosage; Viral Proteins/genetics; Viral Proteins/isolation & purification
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CAUD:D6QY02		GO:0044659: cytolysis by virus of host cell	ECO:0000314:		P		Figure 5 shows Lysgh15 induced lysis in various staphylococcus strains.	complete CACAO 10741
9CAUD:D6QY02	involved_in	GO:0044659: cytolysis by virus of host cell	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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