PMID:20844122

Citation	Datta, S and Desarnaud, F (2010) Protein kinase A in the pedunculopontine tegmental nucleus of rat contributes to regulation of rapid eye movement sleep. J. Neurosci. 30:12263-73
Abstract	Intracellular signaling mechanisms within the pedunculopontine tegmental (PPT) nucleus for the regulation of recovery rapid eye movement (REM) sleep following REM sleep deprivation remain unknown. This study was designed to determine the role of PPT intracellular cAMP-dependent protein kinase A (cAMP-PKA) in the regulation of recovery REM sleep in freely moving rats. The results show that a brief period (3 h) of selective REM sleep deprivation caused REM sleep rebound associated with increased PKA activity and expression of the PKA catalytic subunit protein (PKA-CU) in the PPT. Local application of a cAMP-PKA-activation-selective inhibitor, RpCAMPS (0.55, 1.1, and 2.2 nmol/100 nl; n = 8 rats/group), bilaterally into the PPT, reduced PKA activity and PKA-CU expression in the PPT, and suppressed the recovery REM sleep, in a dose-dependent manner. Regression analyses revealed significant positive relationships between: PPT levels of PKA activity and the total percentages of REM sleep recovery (Rsqr = 0.944; n = 40 rats); PPT levels of PKA-CU expression and the total percentages of REM sleep recovery (Rsqr = 0.937; n = 40 rats); PPT levels of PKA-CU expression and PKA activity (Rsqr = 0.945; n = 40 rats). Collectively, these results provide evidence that activation of intracellular PKA in the PPT contributes to REM sleep recovery following REM sleep deprivation.
Links	PubMed PMC3327880 Online version:10.1523/JNEUROSCI.1563-10.2010
Keywords	Animals; Cyclic AMP-Dependent Protein Kinase Catalytic Subunits/antagonists & inhibitors; Cyclic AMP-Dependent Protein Kinase Catalytic Subunits/physiology; Enzyme Activation/physiology; Intracellular Fluid/enzymology; Male; Neurons/enzymology; Pedunculopontine Tegmental Nucleus/cytology; Pedunculopontine Tegmental Nucleus/enzymology; Protein Kinase Inhibitors/pharmacology; Protein Subunits/physiology; Rats; Rats, Wistar; Recovery of Function/physiology; Sleep Deprivation/enzymology; Sleep Deprivation/physiopathology; Sleep, REM/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:KAPCA	involved_in	GO:0042747: circadian sleep/wake cycle, REM sleep	ECO:0000270: expression pattern evidence used in manual assertion		P		Seeded From UniProt	complete

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