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PMID:20573661
| Citation |
Rajamohan, G, Srinivasan, VB and Gebreyes, WA (2010) Molecular and functional characterization of a novel efflux pump, AmvA, mediating antimicrobial and disinfectant resistance in Acinetobacter baumannii. J. Antimicrob. Chemother. 65:1919-25 |
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| Abstract |
Acinetobacter baumannii has emerged as an important nosocomial pathogen in hospitalized patients, and causes a multitude of infections with significant morbidity and mortality. The aim of this study was to elucidate the role of a novel efflux pump in A. baumannii. |
| Links |
PubMed Online version:10.1093/jac/dkq195 |
| Keywords |
ATP-Binding Cassette Transporters/genetics; ATP-Binding Cassette Transporters/metabolism; Acinetobacter baumannii/drug effects; Acinetobacter baumannii/genetics; Acinetobacter baumannii/metabolism; Anti-Bacterial Agents/metabolism; Anti-Bacterial Agents/pharmacology; Cloning, Molecular; Disinfectants/metabolism; Disinfectants/pharmacology; Drug Resistance, Multiple, Bacterial; Escherichia coli/drug effects; Escherichia coli/genetics; Gene Deletion; Humans; Microbial Sensitivity Tests; Molecular Sequence Data; Mutagenesis, Insertional; Sequence Analysis, DNA; Sequence Homology, Amino Acid |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0015307: drug:proton antiporter activity |
ECO:0000315: |
F |
Figure 1 shows that AmvA confers resistance in E.coli cells carrying the A.baumanii AmvA to antibiotics. Addition of the proton motive force decoupler CCCP renders the bacteria susceptible again. Figure 2 shows that A.baumanii and E.coli carrying the A.baumanii amva gene accumulate ethidium bromide and acriflavine more slowly compared to wild-type E.coli. Once CCCP is added, the concentration of these two increases sharply, implying a proton motive force driven transport of the drugs out of the cell prior to addition of the CCCP. |
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Notes
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