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PMID:20571956
| Citation |
Kim, KM, Lee, SG, Kim, JM, Kim, DS, Song, JY, Kang, HL, Lee, WK, Cho, MJ, Rhee, KH, Youn, HS and Baik, SC (2010) Helicobacter pylori gamma-glutamyltranspeptidase induces cell cycle arrest at the G1-S phase transition. J. Microbiol. 48:372-7 |
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| Abstract |
In our previous study, we showed that Helicobacter pylori gamma-glutamyltranspeptidase (GGT) is associated with H. pylori-induced apoptosis through a mitochondrial pathway. To better understand the role of GGT in apoptosis, we examined the effect of GGT on cell cycle regulation in AGS cells. To determine the effect of recombinant GGT (rGGT) on cell cycle distribution and apoptosis, rGGT-treated and untreated AGS cells were analyzed in parallel by flow cytometry using propidium iodide (PI). We found that rGGT inhibited the growth of AGS cells in a time-dependent manner, and that the pre-exposure of cells to a caspase-3 inhibitor (z-DEVD-fmk) effectively blocked GGT-induced apoptosis. Cell cycle analysis showed G1 phase arrest and apoptosis in AGS cells following rGGT treatment. The rGGT-mediated G1 phase arrest was found to be associated with down-regulation of cyclin E, cyclin A, Cdk 4, and Cdk 6, and the up-regulation of the cyclin-dependent kinase (Cdk) inhibitors p27 and p21. Our results suggest that H. pylori GGT induces cell cycle arrest at the G1-S phase transition. |
| Links |
PubMed Online version:10.1007/s12275-010-9293-8 |
| Keywords |
Apoptosis/drug effects; Apoptosis/physiology; Cell Cycle/drug effects; Cell Cycle/physiology; Cell Cycle Proteins/physiology; Cell Line; Cell Survival/drug effects; Cell Survival/physiology; Epithelial Cells/cytology; Epithelial Cells/drug effects; Epithelial Cells/microbiology; G1 Phase/drug effects; G1 Phase/physiology; Gastric Mucosa/cytology; Gastric Mucosa/drug effects; Gastric Mucosa/microbiology; Helicobacter pylori/enzymology; Helicobacter pylori/pathogenicity; Humans; Recombinant Proteins/pharmacology; S Phase/drug effects; S Phase/physiology; gamma-Glutamyltransferase/pharmacology; gamma-Glutamyltransferase/physiology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:1902807: negative regulation of cell cycle G1/S phase transition |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:1902807: negative regulation of cell cycle G1/S phase transition |
ECO:0000314: |
P |
Figure 2, panels A, B, and C show that when treated with rGGT, the percentage of AGS cells in the G1 phase of the cell cycle is significantly higher than the control. |
complete | ||||
Notes
See also
References
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